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Literature summary for 3.2.1.23 extracted from

  • Goodman, R.E.; Pederson, D.M.
    beta-Galactosidase from Bacillus stearothermophilus (1976), Can. J. Microbiol., 22, 817-825.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
cellobiose 10 mM, 33% inhibition Geobacillus stearothermophilus
Cu2+ 0.1 mM, 92% inhibition Geobacillus stearothermophilus
D-galactose 10 mM, 71% inhibition, product inhibition Geobacillus stearothermophilus
D-glucose 10 mM, 20% inhibition Geobacillus stearothermophilus
Zn2+ 0.1 mM ZnCl2, 30% inhibition Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
lactose
-
Geobacillus stearothermophilus
9.8
-
o-nitrophenyl-beta-D-galactoside
-
Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
215000
-
-
Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Geobacillus stearothermophilus constitutive enzyme ?
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O
-
Geobacillus stearothermophilus D-glucose + D-galactose
-
?
additional information constitutive enzyme Geobacillus stearothermophilus ?
-
?
o-nitrophenyl-beta-D-galactoside + H2O
-
Geobacillus stearothermophilus o-nitrophenol + beta-D-galactose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-life: 20 days Geobacillus stearothermophilus
60
-
10 min, more than 60% loss of activity, 18% loss of activity in presence of 4 mg/ml bovine serum albumin Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.4 hydrolysis of lactose and o-nitrophenyl-beta-D-galactoside Geobacillus stearothermophilus