Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cellobiose | 10 mM, 33% inhibition | Geobacillus stearothermophilus | |
Cu2+ | 0.1 mM, 92% inhibition | Geobacillus stearothermophilus | |
D-galactose | 10 mM, 71% inhibition, product inhibition | Geobacillus stearothermophilus | |
D-glucose | 10 mM, 20% inhibition | Geobacillus stearothermophilus | |
Zn2+ | 0.1 mM ZnCl2, 30% inhibition | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
lactose | - |
Geobacillus stearothermophilus | |
9.8 | - |
o-nitrophenyl-beta-D-galactoside | - |
Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
215000 | - |
- |
Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Geobacillus stearothermophilus | constitutive enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | - |
Geobacillus stearothermophilus | D-glucose + D-galactose | - |
? | |
additional information | constitutive enzyme | Geobacillus stearothermophilus | ? | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Geobacillus stearothermophilus | o-nitrophenol + beta-D-galactose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
half-life: 20 days | Geobacillus stearothermophilus |
60 | - |
10 min, more than 60% loss of activity, 18% loss of activity in presence of 4 mg/ml bovine serum albumin | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.4 | hydrolysis of lactose and o-nitrophenyl-beta-D-galactoside | Geobacillus stearothermophilus |