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Literature summary for 3.2.1.23 extracted from
- beta-Galactosidase from rat mammary gland. Its purification, properties, and role in the biosynthesis of 6beta-O-D-galactopyranosyl myo-inositol (1978), J. Biol. Chem., 253, 3550-3556.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-D-galactosylamine | 50 mM, 78% inhibition | Rattus norvegicus |  |
| Ag+ | 1 mM, complete inhibition | Rattus norvegicus | |
| D-galactono-1,4-lactone | 50 mM, strong inhibition | Rattus norvegicus | |
| D-galactose | 50 mM, 81% inhibition | Rattus norvegicus | |
| D-glucose | 50 mM, 10% inhibition | Rattus norvegicus | |
| Hg2+ | 1 mM, complete inhibition | Rattus norvegicus | |
| PCMB | 1 mM, complete inhibition | Rattus norvegicus | |
| phenyl-beta-D-thiogalactoside | 43 mM, strong inhibition | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.185 | - |
p-nitrophenyl-beta-D-galactoside | - |
Rattus norvegicus | |
| 34 | - |
lactose | transferase reaction, in presence of 400 mM myo-inositol, 1 mg/ml bovine serum albumin, 50 mM glycine/HCl, pH 3.0 | Rattus norvegicus | |
| 345 | - |
myo-inositol | transferase reaction, in presence of 50 mM glycine/HCl buffer, pH 3.0, 80 mM lactose, 0.5 mg/ml alpha-lactalbumin | Rattus norvegicus | |
| 380 | - |
myo-inositol | transferase reaction, in absence of alpha-lactalbumin | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 1 mM, minor stimulation | Rattus norvegicus | |
| Ca2+ | 1 mM, minor stimulation | Rattus norvegicus | |
| Li+ | 50 mM, minor stimulation | Rattus norvegicus | |
| Mn2+ | 1 mM, minor stimulation | Rattus norvegicus | |
| Na+ | 50 mM, minor stimulation | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 110000 | - |
at pH 7.0, gel filtration | Rattus norvegicus |
| 200000 | - |
at pH 5.0, gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mammary gland | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.644 | - |
- |
Rattus norvegicus |
| 1.8 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 4.0, 50 mM sodium citrate buffer, stable for several months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + myo-inositol | - |
Rattus norvegicus | 6-beta-O-D-galactopyranosyl myo-inositol + ? | - |
? | |
| o-nitrophenyl-beta-D-galactoside + myo-inositol | transgalactosylation | Rattus norvegicus | ? | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Rattus norvegicus | p-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl-beta-D-galactoside + myo-inositol | transgalactosylation | Rattus norvegicus | ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.7 | 3 | hydrolysis of p-nitrophenyl-beta-D-galactoside, in glycine/HCl buffer | Rattus norvegicus |
| 3.4 | - |
hydrolysis of p-nitrophenyl-beta-D-galactoside, in sodium citrate buffer | Rattus norvegicus |
| 3.5 | - |
- |
Rattus norvegicus |
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