Literature summary for 3.2.1.23 extracted from

Holmes, E.W.; O'Brien, J.S.
Purification and properties of acid beta-galactosidase from feline liver (1979), Biochemistry, 18, 952-958.

Search for more literature for 3.2.1.23

Inhibitors

Inhibitors	Comment	Organism	Structure
Hg2+	0.2 mM, complete inhibition	Felis catus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.14	-	4-methylumbelliferyl-beta-D-galactoside	-	Felis catus
0.17	-	ganglioside GM1	-	Felis catus
0.62	-	p-nitrophenyl-beta-D-galactoside	-	Felis catus
1.5	-	asialofetuin	-	Felis catus
2.16	-	4-methylumbelliferyl-alpha-L-arabinoside	-	Felis catus
56.9	-	o-nitrophenyl beta-D-fucoside	-	Felis catus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Na+	hydrolysis of 4-methylumbelliferyl-beta-D-galactoside is mildly stimulated by NaCl	Felis catus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
62000	-	2 * 62000, SDS-PAGE of reduced enzyme	Felis catus
115000	-	sucrose density gradient centrifugation	Felis catus

Organism

Organism	UniProt	Comment	Textmining
Felis catus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Felis catus

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Felis catus	-

Storage Stability

Storage Stability	Organism
4°C, protein concentration 1.2 mg/ml, stable for 8 months	Felis catus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl alpha-L-arabinoside + H2O	-	Felis catus	4-methylumbelliferone + alpha-L-arabinose	-	?
4-methylumbelliferyl-beta-D-galactoside + H2O	-	Felis catus	4-methylumbelliferone + beta-D-galactose	-	?
asialofetuin + H2O	-	Felis catus	beta-D-galactose + ?	-	?
glycolipid GM1 ganglioside + H2O	-	Felis catus	beta-D-galactose + ?	-	?
additional information	transfer of galactose from p-nitrophenyl-beta-D-galactoside and ganglioside GM1 to various carbohydrate acceptors	Felis catus	?	-	?
o-nitrophenyl beta-D-fucoside + H2O	-	Felis catus	o-nitrophenol + beta-D-fucose	-	?
p-nitrophenyl-beta-D-galactoside + H2O	-	Felis catus	p-nitrophenol + beta-D-galactose	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 62000, SDS-PAGE of reduced enzyme	Felis catus

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Release 2023.1 (January 2023)