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Literature summary for 3.2.1.23 extracted from
- Characterization of beta-galactosidases of Medicago sativa suspension-cultured cells growing on lactose. Effect of the growth substrate on the activities (1982), Z. Pflanzenphysiol., 106, 401-407.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | inhibition of intracellular enzyme, no inhibition of extracellular enzyme and cell wall enzyme | Medicago sativa |  |
| D-galactose | - |
Medicago sativa | |
| D-glucose | no inhibition | Medicago sativa | |
| EDTA | inhibition of intracellular enzyme, no inhibition of extracellular enzyme and cell wall enzyme | Medicago sativa | |
| Hg2+ | - |
Medicago sativa | |
| lactose | - |
Medicago sativa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | - |
Medicago sativa | 5618 | - |
| extracellular | - |
Medicago sativa | - |
- |
| intracellular | - |
Medicago sativa | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | maximal activation of cell wall enzyme and extracellular enzyme by Na+ and Zn2+ | Medicago sativa | |
| Mg2+ | maximal activation of intracellular enzyme by Mg2+ or Mn2+ | Medicago sativa | |
| Mn2+ | maximal activation of intracellular enzyme by Mg2+ or Mn2+ | Medicago sativa | |
| Zn2+ | maximal activation of cell wall enzyme and extracellular enzyme by Na+ and Zn2+ | Medicago sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | 125000 | various isoenzymes, gel filtration | Medicago sativa |
| 125000 | - |
cell wall enzyme, gel filtration | Medicago sativa |
| 260000 | - |
intracellular enzyme, gel filtration | Medicago sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Medicago sativa | induction by lactose | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Medicago sativa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Medicago sativa | - |
| cell suspension culture | - |
Medicago sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | induction by lactose | Medicago sativa | ? | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Medicago sativa | o-nitrophenol + beta-D-galactose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
cell wall enzyme | Medicago sativa |
| 46 | - |
intracellular enzyme | Medicago sativa |
| 58 | - |
extracellular enzyme | Medicago sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
cell wall enzyme and extracellular enzyme | Medicago sativa |
| 7 | - |
intracellular enzyme | Medicago sativa |
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