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Literature summary for 3.2.1.23 extracted from
- Purification and characterization of a beta-galactosidase from Treponema phagedenis (Reiter strain) (1983), Curr. Microbiol., 8, 341-345.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Treponema phagedenis |  |
| Cu2+ | - |
Treponema phagedenis | |
| Hg2+ | - |
Treponema phagedenis | |
| PCMB | - |
Treponema phagedenis | |
| Zn2+ | - |
Treponema phagedenis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.29 | - |
p-nitrophenyl-beta-D-galactoside | - |
Treponema phagedenis | |
| 0.36 | - |
o-nitrophenyl-beta-D-galactoside | - |
Treponema phagedenis | |
| 5.4 | - |
lactose | - |
Treponema phagedenis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
6 * 90000, SDS-PAGE | Treponema phagedenis |
| 580000 | - |
gel filtration | Treponema phagedenis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Treponema phagedenis | - |
Reiter strain | - |
| Treponema phagedenis Reiter | - |
Reiter strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Treponema phagedenis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1126 | - |
- |
Treponema phagedenis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + H2O | - |
Treponema phagedenis | D-glucose + D-galactose | - |
? | |
| lactose + H2O | - |
Treponema phagedenis Reiter | D-glucose + D-galactose | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Treponema phagedenis | o-nitrophenol + beta-D-galactose | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Treponema phagedenis Reiter | o-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Treponema phagedenis | p-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Treponema phagedenis Reiter | p-nitrophenol + beta-D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 90000, SDS-PAGE | Treponema phagedenis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
hydrolysis of p-nitrophenyl-beta-D-galactoside | Treponema phagedenis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
pH 6.5, 5 min, stable up to | Treponema phagedenis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
hydrolysis of p-nitrophenyl-beta-D-galactoside | Treponema phagedenis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
37°C, 1 h, stable only around | Treponema phagedenis |
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