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Literature summary for 3.2.1.23 extracted from
- Isolation and purification of beta-galactosidase of Lactobacillus murinus CNRZ 313 (1983), Curr. Microbiol., 9, 99-104.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Ligilactobacillus murinus |  |
| dithiothreitol | - |
Ligilactobacillus murinus | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 10 mM CaCl2, 74% inhibition | Ligilactobacillus murinus | |
| Cys | - |
Ligilactobacillus murinus | |
| glutathione | - |
Ligilactobacillus murinus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 10 mM, slight activation | Ligilactobacillus murinus | |
| Mg2+ | 10 mM MgCl2, 3fold higher activity | Ligilactobacillus murinus | |
| Mn2+ | 10 mM, slight activation | Ligilactobacillus murinus | |
| Na+ | slight activation | Ligilactobacillus murinus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 170000 | - |
gel filtration | Ligilactobacillus murinus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ligilactobacillus murinus | - |
CNRZ 131 | - |
| Ligilactobacillus murinus CNRZ 131 | - |
CNRZ 131 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Ligilactobacillus murinus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Ligilactobacillus murinus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Ligilactobacillus murinus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
55% loss of activity after 4 min, 83% loss of activity after 10 min, 33% loss of activity after 4 min in presence of 10 mM MgCl2, 52% loss of activity after 10 min in presence of 10 mM MgCl2 | Ligilactobacillus murinus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
in 50 mM phosphate buffer | Ligilactobacillus murinus |
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