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Literature summary for 3.2.1.23 extracted from

  • Mbuyi-Kalala, A.; Schnek, A.G.; Leonis, J.
    Separation and characterization of four enzyme forms of beta-galactosidase from Saccharomyces lactis (1988), Eur. J. Biochem., 178, 437-443.
    View publication on PubMed
Search for more literature for 3.2.1.23

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 o-nitrophenyl-beta-D-galactoside isoenzyme betaG630 Kluyveromyces lactis
2.5
-
 o-nitrophenyl-beta-D-galactoside isoenzyme betaG550 Kluyveromyces lactis
2.6
-
 o-nitrophenyl-beta-D-galactoside isoenzyme betaG41 Kluyveromyces lactis
12.5
-
 o-nitrophenyl-beta-D-galactoside isoenzyme betaG19 Kluyveromyces lactis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.32 mM, activates isoenzyme betaG550 Kluyveromyces lactis
K+ 0.32 mM KCl, activates isoenzyme betaG550 Kluyveromyces lactis
Mg2+ 0.32 mM MgCl2, activates isoenzyme betaG550 and betaG19 Kluyveromyces lactis
Na+ 0.32 mM NaCl or NaF, activates isoenzyme betaG550 Kluyveromyces lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 isoenzyme betaG19, gel filtration Kluyveromyces lactis
41000
-
 isoenzyme betaG41, gel filtration Kluyveromyces lactis
550000
-
 isoenzyme betaG550, gel filtration Kluyveromyces lactis
630000
-
 isoenzyme betaG630, gel filtration Kluyveromyces lactis

Organism

Organism UniProt Comment Textmining
Kluyveromyces lactis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
4 isoenzymes: betaG630, betaG550, betaG41 and betaG19 Kluyveromyces lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
o-nitrophenyl-beta-D-galactoside + H2O
-
 Kluyveromyces lactis o-nitrophenol + beta-D-galactose
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 o-nitrophenyl-beta-D-galactoside isoenzyme betaG41 Kluyveromyces lactis
5
-
 o-nitrophenyl-beta-D-galactoside isoenzyme betaG630 Kluyveromyces lactis
888
-
 o-nitrophenyl-beta-D-galactoside isoenzyme betaG550 Kluyveromyces lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Kluyveromyces lactis