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Literature summary for 3.2.1.23 extracted from

  • Lind, D.L.; Daniel, R.M.; Cowan, D.A.; Morgan, H.W.
    beta-Galactosidase from a strain of the anaerobic thermophile, Thermoanaerobacter (1989), Enzyme Microb. Technol., 11, 180-186.
No PubMed abstract available
Search for more literature for 3.2.1.23

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 1 mM, activates Thermoanaerobacter sp.
Cys 1 mM, activates Thermoanaerobacter sp.
dithiothreitol 1 mM, activates Thermoanaerobacter sp.
EDTA 1 mM, activates Thermoanaerobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1.5 mM, 45% inhibition Thermoanaerobacter sp.
Co2+ 1.5 mM, complete inhibition Thermoanaerobacter sp.
Cu2+ 1.5 mM, complete inhibition Thermoanaerobacter sp.
Fe2+ 1.5 mM, complete inhibition Thermoanaerobacter sp.
Fe3+ 1.5 mM, complete inhibition Thermoanaerobacter sp.
Mg2+ 1.5 mM, 56% inhibition Thermoanaerobacter sp.
Ni2+ 1.5 mM, complete inhibition Thermoanaerobacter sp.
PCMB
-
 Thermoanaerobacter sp.
phenylmethylsulfonyl fluoride
-
 Thermoanaerobacter sp.
Zn2+ 1.5 mM, complete inhibition Thermoanaerobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3
-
 o-nitrophenyl-beta-D-galactoside
-
 Thermoanaerobacter sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
 Thermoanaerobacter sp. 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 0.1 M, activates Thermoanaerobacter sp.
Li+ 0.1 M, activates Thermoanaerobacter sp.
Na+ 0.1 M, activates Thermoanaerobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
154000
-
 gel filtration Thermoanaerobacter sp.

Organism

Organism UniProt Comment Textmining
Thermoanaerobacter sp.
-
-
-
Thermoanaerobacter sp. TP6-B1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermoanaerobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
99
-
 at 70°C Thermoanaerobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O
-
 Thermoanaerobacter sp. D-glucose + D-galactose
-
 ?
lactose + H2O
-
 Thermoanaerobacter sp. TP6-B1 D-glucose + D-galactose
-
 ?
methyl O-beta-galactoside + H2O
-
 Thermoanaerobacter sp. methanol + beta-D-galactose
-
 ?
methyl O-beta-galactoside + H2O
-
 Thermoanaerobacter sp. TP6-B1 methanol + beta-D-galactose
-
 ?
methylthio-beta-D-galactoside + H2O
-
 Thermoanaerobacter sp. galactose + methanethiol
-
 ?
methylthio-beta-D-galactoside + H2O
-
 Thermoanaerobacter sp. TP6-B1 galactose + methanethiol
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Thermoanaerobacter sp. o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Thermoanaerobacter sp. TP6-B1 o-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl alpha-L-arabinoside + H2O
-
 Thermoanaerobacter sp. p-nitrophenol + alpha-L-arabinose
-
 ?
p-nitrophenyl alpha-L-arabinoside + H2O
-
 Thermoanaerobacter sp. TP6-B1 p-nitrophenol + alpha-L-arabinose
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 half-life: 220 min Thermoanaerobacter sp.
75
-
 half-life: 155 min Thermoanaerobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
431
-
 o-nitrophenyl-beta-D-galactoside
-
 Thermoanaerobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 hydrolysis of o-nitrophenyl-beta-D-galactoside Thermoanaerobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.2 7.6 more than 50% of maximal activity at pH 5.2 and at pH 7.6 Thermoanaerobacter sp.