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Literature summary for 3.2.1.23 extracted from

  • Tulsiani, D.R.P.; Skudlarek, M.D.; Araki, Y.; Orgebin-Christ, M.C.
    Purification and characterization of two forms of beta-D-galactosidase from rat epididymal luminal fluid: evidence for their role in the modification of sperm plasma membrane glycoproteins (1995), Biochem. J., 305, 41-50.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.23

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
 p-nitrophenyl-beta-D-galactoside 97000 da enzyme form and 84000 Da enzyme form Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
 x * 84000, and a second enzyme form of MW 97000 Da, SDS-PAGE Rattus norvegicus
97000
-
 x * 97000, and a second enzyme form of MW 84000 Da, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus degalactosylation of peanut agglutinin-positive glycoprotein of MW 130000-150000 Da, present in the caput sperm ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the two enzyme forms mainly contain high mannose/hybrid-type, and biantennary complex-type oligosaccharides. The 84000 Da enzyme form contains 13.4 mg sugar per 100 mg enzyme, the 97000 Da enzyme form contains 28.3 mg sugar per 100 mg of enzyme, the 84000 Da enzyme form contains 1.9 mg of N-acetylneuraminic acid per 100 mg enzyme, the 97000 Da enzyme form contains 6.3 mg of N-acetylneuraminic acid per 100 mg enzyme Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
a 84000 Da enzyme form and a 97000 Da enzyme form Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
epididymis epididymal luminal fluid Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Storage Stability

Storage Stability Organism
-20°C, complete inactivation after a few days Rattus norvegicus
0-4°C, 0.2-0.3 mg of protein per ml, 10 mM Tris/HCl buffer, 0.1 M NaCl, 0.02% sodium azide, both enzyme forms, stable for 3 months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
galactosyl-fetuin + H2O
-
 Rattus norvegicus galactose + fetuin
-
 ?
galactosyl-ovomucoid + H2O
-
 Rattus norvegicus galactose + ovomucoid
-
 ?
additional information degalactosylation of peanut agglutinin-positive glycoprotein of MW 130000-150000 Da, present in the caput sperm Rattus norvegicus ?
-
 ?
p-nitrophenyl-beta-D-galactoside + H2O
-
 Rattus norvegicus p-nitrophenol + beta-D-galactose
-
 ?

Subunits

Subunits Comment Organism
? x * 84000, and a second enzyme form of MW 97000 Da, SDS-PAGE Rattus norvegicus
? x * 97000, and a second enzyme form of MW 84000 Da, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 hydrolysis of p-nitrophenyl-beta-D-galactoside Rattus norvegicus
6.4 6.8 hydrolysis of galactosyl-fetuin Rattus norvegicus