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Literature summary for 3.2.1.22 extracted from

  • E, S.; Potumarthi, R.; A, N.; Mangamoori, L.
    Purification and characterisation of intracellular alpha-galactosidases from Acinetobacter sp. (2015), 3 Biotech, 5, 925-932 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.22

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 isoform Ag-I shows 110% activity at 1% (v/v) Triton X-100 Acinetobacter sp.
Tween 20 isoform Ag-I shows 128% activity and isoform at 1% (v/v) Tween 20 Acinetobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol isoform Ag-I shows 88% residual activity and isoform Ag-II shows 74% residual activity at 5 mM 2-mercaptoethanol Acinetobacter sp.
Ag+ isoform Ag-I shows 7% residual activity and isoform Ag-II is completely inhibited by 5 mM Ag+ Acinetobacter sp.
Ca2+ isoform Ag-I shows 90% residual activity and isoform Ag-II shows 67% residual activity at 5 mM Ca2+ Acinetobacter sp.
Cu2+ isoform Ag-I shows 80% residual activity and isoform Ag-II shows 56% residual activity at 5 mM Cu2+ Acinetobacter sp.
D-galactose isoform Ag-I shows 92% residual activity and isoform Ag-II shows 72% residual activity at 5 mM D-galactose Acinetobacter sp.
D-glucose isoform Ag-I shows 82% residual activity and isoform Ag-II shows 75% residual activity at 5 mM D-glucose Acinetobacter sp.
Hg2+ isoform Ag-I shows 5% residual activity and isoform Ag-II is completely inhibited by 5 mM Hg2+ Acinetobacter sp.
K+ isoform Ag-I shows 98% residual activity and isoform Ag-II shows 67% residual activity at 5 mM K+ Acinetobacter sp.
lactose isoform Ag-I shows 74% residual activity and isoform Ag-II shows 70% residual activity at 5 mM lactose Acinetobacter sp.
additional information not inhibited by EDTA Acinetobacter sp.
p-chloromercuribenzoate complete inhibition at 5 mM Acinetobacter sp.
SDS complete inhibition at 1% (w/v) Acinetobacter sp.
stachyose isoform Ag-I shows 71% residual activity and isoform Ag-II shows 63% residual activity at 5 mM D-stachyose Acinetobacter sp.
Urea isoform Ag-II shows 92% residual activity at 5 mM urea Acinetobacter sp.
Zn2+ isoform Ag-I shows 90% residual activity and isoform Ag-II shows 75% residual activity at 5 mM Zn2+ Acinetobacter sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ isoform Ag-I shows 105% activity at 5 mM Mg2+ Acinetobacter sp.
Mg2+ isoform Ag-I shows 108% activity at 5 mM Mg2+ Acinetobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
raffinose + H2O Acinetobacter sp.
-
 sucrose + D-galactose
-
 ?

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephacryl S-300 gel filtration Acinetobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7
-
 crude extract, at pH 7.0 and 36°C Acinetobacter sp.
8
-
 isoform Ag-II, after 4.7fold purification, at pH 7.0 and 36°C Acinetobacter sp.
12
-
 isoform Ag-I, after 7fold purification, at pH 7.0 and 36°C Acinetobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Acinetobacter sp. 4-nitrophenol + alpha-D-galactopyranose
-
 ?
raffinose + H2O
-
 Acinetobacter sp. sucrose + D-galactose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 37000, isoform Ag-II, SDS-PAGE Acinetobacter sp.
monomer 1 * 65000, isoform Ag-I, SDS-PAGE Acinetobacter sp.

Synonyms

Synonyms Comment Organism
Ag-I isoform Acinetobacter sp.
Ag-II isoform Acinetobacter sp.
alpha-galactosidase I isoform Acinetobacter sp.
alpha-galactosidase II isoform Acinetobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 isoform Ag-II Acinetobacter sp.
50 60 isoform Ag-I Acinetobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 90 the half-life of isoform Ag-I varies from 1.85 h at 90°C to 7.6 h at 70°C Acinetobacter sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 raffinose isoform Ag-II, at pH 7.0 and 36°C Acinetobacter sp.
1.83
-
 raffinose isoform Ag-I, at pH 7.0 and 36°C Acinetobacter sp.
2.53
-
 4-nitrophenyl alpha-D-galactopyranoside isoform Ag-II, at pH 7.0 and 36°C Acinetobacter sp.
7.42
-
 4-nitrophenyl alpha-D-galactopyranoside isoform Ag-I, at pH 7.0 and 36°C Acinetobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 isoform Ag-I is highly active and stable over broad pH range showing more than 60% activity up to pH 9.0 with low activities at acidic pH. After 24 h of incubation at 36°C, isoform Ag-I retains 85% enzyme activity at pH 7.0, whereas isoform Ag-II retains 52% activity Acinetobacter sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.55
-
 raffinose isoform Ag-I, at pH 7.0 and 36°C Acinetobacter sp.
1.35
-
 raffinose isoform Ag-II, at pH 7.0 and 36°C Acinetobacter sp.
3.72
-
 4-nitrophenyl alpha-D-galactopyranoside isoform Ag-II, at pH 7.0 and 36°C Acinetobacter sp.
22.47
-
 4-nitrophenyl alpha-D-galactopyranoside isoform Ag-I, at pH 7.0 and 36°C Acinetobacter sp.