Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | isoform Ag-I shows 110% activity at 1% (v/v) Triton X-100 | Acinetobacter sp. | |
Tween 20 | isoform Ag-I shows 128% activity and isoform at 1% (v/v) Tween 20 | Acinetobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | isoform Ag-I shows 88% residual activity and isoform Ag-II shows 74% residual activity at 5 mM 2-mercaptoethanol | Acinetobacter sp. | |
Ag+ | isoform Ag-I shows 7% residual activity and isoform Ag-II is completely inhibited by 5 mM Ag+ | Acinetobacter sp. | |
Ca2+ | isoform Ag-I shows 90% residual activity and isoform Ag-II shows 67% residual activity at 5 mM Ca2+ | Acinetobacter sp. | |
Cu2+ | isoform Ag-I shows 80% residual activity and isoform Ag-II shows 56% residual activity at 5 mM Cu2+ | Acinetobacter sp. | |
D-galactose | isoform Ag-I shows 92% residual activity and isoform Ag-II shows 72% residual activity at 5 mM D-galactose | Acinetobacter sp. | |
D-glucose | isoform Ag-I shows 82% residual activity and isoform Ag-II shows 75% residual activity at 5 mM D-glucose | Acinetobacter sp. | |
Hg2+ | isoform Ag-I shows 5% residual activity and isoform Ag-II is completely inhibited by 5 mM Hg2+ | Acinetobacter sp. | |
K+ | isoform Ag-I shows 98% residual activity and isoform Ag-II shows 67% residual activity at 5 mM K+ | Acinetobacter sp. | |
lactose | isoform Ag-I shows 74% residual activity and isoform Ag-II shows 70% residual activity at 5 mM lactose | Acinetobacter sp. | |
additional information | not inhibited by EDTA | Acinetobacter sp. | |
p-chloromercuribenzoate | complete inhibition at 5 mM | Acinetobacter sp. | |
SDS | complete inhibition at 1% (w/v) | Acinetobacter sp. | |
stachyose | isoform Ag-I shows 71% residual activity and isoform Ag-II shows 63% residual activity at 5 mM D-stachyose | Acinetobacter sp. | |
Urea | isoform Ag-II shows 92% residual activity at 5 mM urea | Acinetobacter sp. | |
Zn2+ | isoform Ag-I shows 90% residual activity and isoform Ag-II shows 75% residual activity at 5 mM Zn2+ | Acinetobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | isoform Ag-I shows 105% activity at 5 mM Mg2+ | Acinetobacter sp. | |
Mg2+ | isoform Ag-I shows 108% activity at 5 mM Mg2+ | Acinetobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
raffinose + H2O | Acinetobacter sp. | - |
sucrose + D-galactose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Sephacryl S-300 gel filtration | Acinetobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.7 | - |
crude extract, at pH 7.0 and 36°C | Acinetobacter sp. |
8 | - |
isoform Ag-II, after 4.7fold purification, at pH 7.0 and 36°C | Acinetobacter sp. |
12 | - |
isoform Ag-I, after 7fold purification, at pH 7.0 and 36°C | Acinetobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Acinetobacter sp. | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
raffinose + H2O | - |
Acinetobacter sp. | sucrose + D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37000, isoform Ag-II, SDS-PAGE | Acinetobacter sp. |
monomer | 1 * 65000, isoform Ag-I, SDS-PAGE | Acinetobacter sp. |
Synonyms | Comment | Organism |
---|---|---|
Ag-I | isoform | Acinetobacter sp. |
Ag-II | isoform | Acinetobacter sp. |
alpha-galactosidase I | isoform | Acinetobacter sp. |
alpha-galactosidase II | isoform | Acinetobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
isoform Ag-II | Acinetobacter sp. |
50 | 60 | isoform Ag-I | Acinetobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 90 | the half-life of isoform Ag-I varies from 1.85 h at 90°C to 7.6 h at 70°C | Acinetobacter sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
raffinose | isoform Ag-II, at pH 7.0 and 36°C | Acinetobacter sp. | |
1.83 | - |
raffinose | isoform Ag-I, at pH 7.0 and 36°C | Acinetobacter sp. | |
2.53 | - |
4-nitrophenyl alpha-D-galactopyranoside | isoform Ag-II, at pH 7.0 and 36°C | Acinetobacter sp. | |
7.42 | - |
4-nitrophenyl alpha-D-galactopyranoside | isoform Ag-I, at pH 7.0 and 36°C | Acinetobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | isoform Ag-I is highly active and stable over broad pH range showing more than 60% activity up to pH 9.0 with low activities at acidic pH. After 24 h of incubation at 36°C, isoform Ag-I retains 85% enzyme activity at pH 7.0, whereas isoform Ag-II retains 52% activity | Acinetobacter sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
raffinose | isoform Ag-I, at pH 7.0 and 36°C | Acinetobacter sp. | |
1.35 | - |
raffinose | isoform Ag-II, at pH 7.0 and 36°C | Acinetobacter sp. | |
3.72 | - |
4-nitrophenyl alpha-D-galactopyranoside | isoform Ag-II, at pH 7.0 and 36°C | Acinetobacter sp. | |
22.47 | - |
4-nitrophenyl alpha-D-galactopyranoside | isoform Ag-I, at pH 7.0 and 36°C | Acinetobacter sp. |