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Literature summary for 3.2.1.22 extracted from

  • Simila, J.; Gernig, A.; Murray, P.; Fernandes, S.; Tuohy, M.G.
    Cloning and expression of a thermostable alpha-galactosidase from the thermophilic fungus Talaromyces emersonii in the methylotrophic yeast Pichia pastoris (2010), J. Microbiol. Biotechnol., 20, 1653-1663.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris as a His-tagged fusion protein Rasamsonia emersonii

Inhibitors

Inhibitors Comment Organism Structure
galactose
-
Rasamsonia emersonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
4-nitrophenyl-alpha-D-galactopyranoside pH 4.5, 50°C Rasamsonia emersonii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
SDS-PAGE after treatment with endoglycosidase, recombinant protein Rasamsonia emersonii
110000
-
SDS-PAGE, recombinant protein Rasamsonia emersonii

Organism

Organism UniProt Comment Textmining
Rasamsonia emersonii
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Rasamsonia emersonii

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography Rasamsonia emersonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-D-galactopyranoside + H2O
-
Rasamsonia emersonii 4-nitrophenol + alpha-D-galactopyranose
-
?
carob galactomannan + H2O
-
Rasamsonia emersonii ?
-
?
di-galactomannopentose + H2O
-
Rasamsonia emersonii ?
-
?
galactomannotriose + H2O
-
Rasamsonia emersonii ?
-
?
guar gum + H2O
-
Rasamsonia emersonii ?
-
?
locust bean gum + H2O
-
Rasamsonia emersonii ?
-
?
melibiose + H2O
-
Rasamsonia emersonii alpha-D-galactose + alpha-D-glucose
-
?
raffinose + H2O
-
Rasamsonia emersonii sucrose + alpha-D-galactose
-
?
stachyose + 2 H2O
-
Rasamsonia emersonii 2 alpha-D-galactose + sucrose
-
?

Subunits

Subunits Comment Organism
monomer
-
Rasamsonia emersonii

Synonyms

Synonyms Comment Organism
alpha-gal1
-
Rasamsonia emersonii
alpha-galactosidase
-
Rasamsonia emersonii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Rasamsonia emersonii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 70
-
Rasamsonia emersonii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
no loss in activity after 10 days at 50°C Rasamsonia emersonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Rasamsonia emersonii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.77
-
galactose pH 4.5, 50°C Rasamsonia emersonii