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Literature summary for 3.2.1.22 extracted from
- Aspergillus nidulans alpha-galactosidase of glycoside hydrolase family 36 catalyses the formation of alpha-galacto-oligosaccharides by transglycosylation (2010), FEBS J., 277, 3538-3551.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Aspergillus nidulans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.27 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 5, 37°C | Aspergillus nidulans |  |
| 12 | - |
melibiose | pH 5, 37°C | Aspergillus nidulans | |
| 15 | - |
raffinose | pH 5, 37°C | Aspergillus nidulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83000 | - |
SDS-PAGE | Aspergillus nidulans |
| 335000 | - |
gel filtration | Aspergillus nidulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl-alpha-D-galactopyranoside + H2O | - |
Aspergillus nidulans | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
| melibiose + H2O | - |
Aspergillus nidulans | D-galactose + D-glucose | - |
? | |
| raffinose + H2O | - |
Aspergillus nidulans | sucrose + alpha-D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 83000, SDS-PAGE | Aspergillus nidulans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AglC | - |
Aspergillus nidulans |
| alpha-galactosidase | - |
Aspergillus nidulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus nidulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
retains more than 95% activity after 15 min incubation up to 45°C at pH 5.0 | Aspergillus nidulans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 586 | - |
raffinose | pH 5, 37°C | Aspergillus nidulans | |
| 1067 | - |
melibiose | pH 5, 37°C | Aspergillus nidulans | |
| 1278 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 5, 37°C | Aspergillus nidulans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus nidulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.6 | 9.9 | - |
Aspergillus nidulans |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 39.1 | - |
raffinose | pH 5, 37°C | Aspergillus nidulans | |
| 88.9 | - |
melibiose | pH 5, 37°C | Aspergillus nidulans | |
| 4730 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 5, 37°C | Aspergillus nidulans | |
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Release 2023.1 (January 2023)
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