Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli TOP10 or XL1 cells | Pseudoalteromonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80055 | - |
2 * 80055, calculated from amino acid sequence | Pseudoalteromonas sp. |
80300 | - |
2 * 80300, SDS-PAGE | Pseudoalteromonas sp. |
170000 | - |
gel filtration | Pseudoalteromonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
melibiose + H2O | Pseudoalteromonas sp. | - |
D-galactose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas sp. | Q19AX0 | VKM B-2135 D | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE Toyopearl column chromatography, Sepharose CL-4B column chromatography, and Source 15Q PE gel filtration | Pseudoalteromonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.11 | - |
crude extract, using 4-nitrophenyl alpha-D-galactopyranoside as substrate, in 0.1 M Na+-phosphate buffer (pH 7.3), 0.15 M NaCl at 20°C | Pseudoalteromonas sp. |
231 | - |
after 2100fold purification, using 4-nitrophenyl alpha-D-galactopyranoside as substrate, in 0.1 M Na+-phosphate buffer (pH 7.3), 0.15 M NaCl at 20°C | Pseudoalteromonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-galactopyranoside + H2O | highest activity | Pseudoalteromonas sp. | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | low activity | Pseudoalteromonas sp. | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
melibiose + H2O | - |
Pseudoalteromonas sp. | D-galactose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 80055, calculated from amino acid sequence | Pseudoalteromonas sp. |
homodimer | 2 * 80300, SDS-PAGE | Pseudoalteromonas sp. |
Synonyms | Comment | Organism |
---|---|---|
alpha-D-galactosidase | - |
Pseudoalteromonas sp. |
Alpha-D-galactoside galactohydrolase | - |
Pseudoalteromonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 22 | - |
Pseudoalteromonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | 7.7 | - |
Pseudoalteromonas sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudoalteromonas sp. | calculated from amino acid sequence | - |
5.6 |