Cloned (Comment) | Organism |
---|---|
expressed in Trichoplusia ni insect cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 25% (w/v) PEG 4000, 200 mM (NH4)2SO4, and 100 mM NaCH3COO, pH 4.6, at 20°C | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D170A | the mutant protein lacks the active site nucleophile | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Homo sapiens | 5764 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
melibiose + H2O | Homo sapiens | - |
D-galactose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P06280 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-galactoside + H2O | - |
Homo sapiens | 4-nitrophenol + alpha-D-galactose | - |
? | |
melibiose + H2O | - |
Homo sapiens | D-galactose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
alpha-Gal | - |
Homo sapiens |
alpha-Gal A | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | defects in human alpha-GAL lead to the development of Fabry disease, a lysosomal storage disorder characterized by the buildup of alpha-galactosylated substrates in the tissues | Homo sapiens |