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Literature summary for 3.2.1.22 extracted from
- Induction and characterization of an unusual alpha-D-galactosidase from Talaromyces flavus (2007), J. Biotechnol., 128, 61-71.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 6-deoxy-D-glucose | the enzyme is produced exclusively in the presence of the specific inducer 6-deoxy-D-glucose, also named quinovose | Talaromyces flavus |  |
| additional information | D-galactose, melibiose, raffinose, stachyose, methyl alpha-D-galactopyranoside, 6-deoxy-D-galactose, D-glucose, 2-deoxy D-glucose, 6-O-tosyl-D-glucose, 6-chloro-6-deoxy-dglucose, D-arabinose, and D-xylose do not stimulate enzyme production | Talaromyces flavus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-D-galactopyranosyl azide | 75% inhibition at 2 mM | Talaromyces flavus | |
| D-galactose | 51% inhibition at 2 mM | Talaromyces flavus | |
| D-xylose | 49% inhibition at 2 mM | Talaromyces flavus | |
| lactose | 12% inhibition at 2 mM | Talaromyces flavus | |
| melibiose | 46% inhibition at 2 mM | Talaromyces flavus | |
| methyl alpha-D-galactopyranoside | 15% inhibition at 2 mM | Talaromyces flavus | |
| methyl beta-D-galactopyranoside | 13% inhibition at 2 mM | Talaromyces flavus | |
| additional information | not inhibited by 2-acetamido-2-deoxy-D-galactopyranose, D-galactosamine hydrochloride, D-glucose, 2-deoxy-D-glucose, 6-deoxy-D-glucose, 2-acetamido-2-deoxy-D-glucopyranose, D-glucosamine hydrochloride, saccharose, stachyose, D-fructose and D-arabinose | Talaromyces flavus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.54 | - |
p-nitrophenyl alpha-D-galactopyranoside | at 30°C | Talaromyces flavus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
SDS-PAGE | Talaromyces flavus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Talaromyces flavus | - |
- |
- |
| Talaromyces flavus CCF2686 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE column chromatography and Fractogel SO3- gel filtration | Talaromyces flavus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.75 | - |
unpurified enzyme from culture broth | Talaromyces flavus |
| 619 | - |
after 108fold purification | Talaromyces flavus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guar gum + H2O | - |
Talaromyces flavus | ? | - |
? | |
| guar gum + H2O | - |
Talaromyces flavus CCF2686 | ? | - |
? | |
| locust bean gum + H2O | - |
Talaromyces flavus | ? | - |
? | |
| locust bean gum + H2O | - |
Talaromyces flavus CCF2686 | ? | - |
? | |
| melibiose + H2O | negligible hydrolytic activity towards melibiose | Talaromyces flavus | D-galactose + D-glucose | - |
? | |
| melibiose + H2O | negligible hydrolytic activity towards melibiose | Talaromyces flavus CCF2686 | D-galactose + D-glucose | - |
? | |
| p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Talaromyces flavus | p-nitrophenol + alpha-D-galactose | - |
? | |
| p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Talaromyces flavus CCF2686 | p-nitrophenol + alpha-D-galactose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-D-galactopyranoside galactohydrolase | - |
Talaromyces flavus |
| alpha-D-galactosidase | - |
Talaromyces flavus |
| alphaGal1 | - |
Talaromyces flavus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Talaromyces flavus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | the enzyme remains fully stable for 22 h at temperatures up to 40°C and is inactivated after 5 h at temperatures above 60°C | Talaromyces flavus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 4.5 | - |
Talaromyces flavus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
alpha-D-galactopyranosyl azide | at 30°C | Talaromyces flavus | |
| 0.38 | - |
D-galactose | at 30°C | Talaromyces flavus | |
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