Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.22 extracted from

  • Gote, M.M.; Khan, M.I.; Khire, J.M.
    Active site directed chemical modification of alpha-galactosidase from Bacillus stearothermophilus (NCIM 5146): Involvement of lysine, tryptophan and carboxylate residues in catalytic site (2007), Enzyme Microb. Technol., 40, 1312-1320.
No PubMed abstract available
Search for more literature for 3.2.1.22

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide 31% residual activity at 100 mM, at pH 6.0 Geobacillus stearothermophilus
2,4,6-Trinitrobenzenesulfonic acid 20% residual activity at 2 mM, at pH 8.4 Geobacillus stearothermophilus
citraconic anhydride 30% residual activity at 5 mM, at pH 7.8 Geobacillus stearothermophilus
diethyldicarbonate complete inhibition at 2 mM, at pH 6.0 Geobacillus stearothermophilus
N-bromosuccinimide complete inhibition at 0.02 mM, at pH 5.0 Geobacillus stearothermophilus
p-chloromercuribenzoate 16% residual activity at 0.01 mM, at pH 5.8 Geobacillus stearothermophilus
Woodward's reagent K 21% residual activity at 5 mM, at pH 6.0 Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Geobacillus stearothermophilus NCIM 5146
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Geobacillus stearothermophilus p-nitrophenol + alpha-D-galactose
-
 ?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Geobacillus stearothermophilus NCIM 5146 p-nitrophenol + alpha-D-galactose
-
 ?

Synonyms

Synonyms Comment Organism
Alpha-D-galactoside galactohydrolase
-
 Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5
-
 Geobacillus stearothermophilus