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Literature summary for 3.2.1.22 extracted from
- Expression of rice (Oryza sativa L. var. Nipponbare) alpha-galactosidase genes in Escherichia coli and characterization (2007), Biosci. Biotechnol. Biochem., 71, 520-526.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain Origami (DE) | Oryza sativa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | alpha-Gal II, 67% decrease of activity at 1 mM; alpha-Gal III, 84% decrease of activity at 1 mM | Oryza sativa |  |
| Hg2+ | alpha-Gal II, 93% decrease of activity at 1 mM; alpha-Gal III, 87% decrease of activity at 1 mM | Oryza sativa | |
| p-chloromercuribenzoate | alpha-Gal III shows less than 8% of the original activity by treatment with 0.1 mM p-chloromercuribenzoate at 20°C for 1 h; alpha-Gal II shows less than 6% of the original activity by treatment with 0.1 mM p-chloromercuribenzoate at 20°C for 1 h | Oryza sativa | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Ca2+, Cu2+, Fe2+, Mg2+, Mn2+, Ni2+, and Zn2+ do not affect activity | Oryza sativa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41020 | - |
calculated from amino acid sequence | Oryza sativa |
| 41520 | - |
calculated from amino acid sequence | Oryza sativa |
| 42000 | - |
SDS-PAGE | Oryza sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | Q7XIV4 | variant Nipponbare | - |
| Oryza sativa | Q9FXT4 | alpha-Gal II; variant Nipponbare | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-Sepharose column chromatography | Oryza sativa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.31 | - |
alpha-Gal III, crude extract | Oryza sativa |
| 2.25 | - |
alpha-Gal II, crude extract | Oryza sativa |
| 9.13 | - |
alpha-Gal III, after 29.5fold purification | Oryza sativa |
| 52.8 | - |
alpha-Gal II, after 23.5fold purification | Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6(1)-alpha-D-galactosyl mannobiose + H2O | 2.6% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
| 6(1)-alpha-D-galactosyl mannobiose + H2O | 6.8% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
| 6(1)-alpha-D-galactosyl mannotriose + H2O | 2.7% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
| 6(1)-alpha-D-galactosyl mannotriose + H2O | 6.9% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
| 6(3),6(4)-di-alpha-D-galactosyl mannopentaose + H2O | 0.5% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
| 6(3),6(4)-di-alpha-D-galactosyl mannopentaose + H2O | 7.3% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
| guar gum + H2O | 0.4% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
| guar gum + H2O | 1.7% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
| locust bean gum + H2O | 0.4% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
| locust bean gum + H2O | 1.4% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | ? | - |
? | |
| melibiose + H2O | 1.2% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | D-galactose + D-glucose | - |
? | |
| melibiose + H2O | 6.9% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | D-galactose + D-glucose | - |
? | |
| p-nitrophenyl alpha-D-galactoside + H2O | 100% activity | Oryza sativa | p-nitrophenol + alpha-D-galactose | - |
? | |
| raffinose + H2O | 1.89% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | sucrose + alpha-D-galactose | - |
? | |
| raffinose + H2O | 10.1% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | sucrose + alpha-D-galactose | - |
? | |
| stachyose + 2 H2O | 0.47% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | 2 alpha-D-galactose + sucrose | - |
? | |
| stachyose + 2 H2O | 1.9% relative activity compared to p-nitrophenyl alpha-D-galactoside | Oryza sativa | 2 alpha-D-galactose + sucrose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-Gal II | - |
Oryza sativa |
| alpha-Gal III | - |
Oryza sativa |
| alpha-galactosidase II | - |
Oryza sativa |
| alpha-galactosidase III | - |
Oryza sativa |
| alpha-galactoside galactohydrolase | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Oryza sativa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
alpha-Gal II is stable for 1 h up to 35°C at pH 5.0 | Oryza sativa |
| 35 | - |
alpha-Gal III is stable for 1 h up to 20°C at pH 5.0 | Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Oryza sativa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 8.5 | alpha-Gal II is stable at pH 3.5-8.5 at 25°C | Oryza sativa |
| 4 | 7 | alpha-Gal III is stable at pH 4.0-7.0 at 20°C | Oryza sativa |
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