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Literature summary for 3.2.1.22 extracted from

  • Guimaraes, V.M.; Tavares de Rezende, S.; Moreira, M.A.; Goncalves de Barros, E.; Felix, C.R.
    Characterization of alpha-galactosidases from germinating soybean seed and their use for hydrolysis of oligosaccharides (2001), Phytochemistry, 58, 67-73.
    View publication on PubMed
Search for more literature for 3.2.1.22

Inhibitors

Inhibitors Comment Organism Structure
alpha-D-galactose 2 mM, 17% inhibition of enzyme P1, 68.5% inhibition of enzyme P2 Glycine max
CuSO4 2 mM, 94.5% inhibition of enzyme P1, 48% inhibition of enzyme P2 Glycine max
galactose
-
 Glycine max
melibiose 2 mM, 16% inhibition of enzyme P1, 36% inhibition of enzyme P2 Glycine max
SDS 1 mM, 98% inhibition of enzyme P1, 37% inhibition of enzyme P2 Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.76
-
 p-nitrophenyl alpha-D-galactopyranoside enzyme form P2 Glycine max
1.55
-
 p-nitrophenyl alpha-D-galactopyranoside enzyme form P1 Glycine max
5.34
-
 raffinose enzyme form P2 Glycine max
5.53
-
 melibiose enzyme form P2 Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 x * 33000, enzyme form P1, SDS-PAGE Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
-
-
-

Purification (Commentary)

Purification (Comment) Organism
one of the enzyme preparations, P1, shows a single protein with 33000 Da and the second preparation, P2, has two proteins with 31000 Da and 33000 Da Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
seed germinating Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.2
-
 enzyme form P1 Glycine max
62.5
-
 enzyme form P2 Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O enzyme P1: 6.2% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 1.3% of the activity with p-nitrophenyl alpha-D-galactopyranoside Glycine max beta-D-galactose + D-glucose
-
 ?
maltose + H2O enzyme P1: 4% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 1.6% of the activity with p-nitrophenyl alpha-D-galactopyranoside Glycine max alpha-D-glucose + D-glucose
-
 ?
melibiose + H2O enzyme P1: 6.9% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 10% of the activity with p-nitrophenyl alpha-D-galactopyranoside Glycine max alpha-D-galactose + D-glucose
-
 ?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Glycine max p-nitrophenol + alpha-D-galactose
-
 ?
p-nitrophenyl beta-D-galactopyranoside + H2O enzyme P1: 5.5% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: no activity Glycine max p-nitrophenol + alpha-D-galactose
-
 ?
raffinose + H2O enzyme P1: 11.8% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 54.3% of the activity with p-nitrophenyl alpha-D-galactopyranoside Glycine max sucrose + alpha-D-galactose
-
 ?
stachyose + 2 H2O enzyme P1: 5% of the activity with p-nitrophenyl alpha-D-galactopyranoside. Enzyme P2: 10.5% of the activity with p-nitrophenyl alpha-D-galactopyranoside Glycine max 2 alpha-D-galactose + sucrose
-
 ?

Subunits

Subunits Comment Organism
? x * 33000, enzyme form P1, SDS-PAGE Glycine max
More one of the enzyme preparations, P1, shows a single protein with 33000 Da and the second preparation, P2, has two proteins with 31000 Da and 33000 Da Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 enzyme form P1 Glycine max
50
-
 enzyme form P2 Glycine max

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 about 50% of maximal activity at 30°C and at 70°C, enzyme P1 Glycine max
35 55 35°C: about 40% of maximal activity, 55°C: about 40% of maximal activity, enzyme P2 Glycine max

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 2 h, both enzymes retain about 50% of their original activity Glycine max
50
-
 45 min, 90% loss of activity of enzyme P1 and P2 Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 enzyme form P1 Glycine max
5.5
-
 enzyme form P2 Glycine max

pH Range

pH Minimum pH Maximum Comment Organism
3 6.5 pH 3.0: enzyme P1 shows about 60% of maximal activity, enzyme P2 shows about 70% of maximal activity, pH 6.5: enzyme p1 shows about 30% of maximal activity, enzyme P2 shows about 75% of maximal activity Glycine max

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.65
-
 galactose enzyme form P2 Glycine max