Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga neapolitana |
expression in Escherichia coli | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuCl2 | 1 mM, 50% inhibition | Thermotoga maritima | |
FeCl2 | 1 mM, 50% inhibition | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.075 | - |
p-nitrophenyl alpha-D-galactopyranoside | pH 5, 75°C | Thermotoga maritima | |
0.74 | - |
p-nitrophenyl alpha-D-galactopyranoside | pH 7, 98°C | Thermotoga neapolitana | |
2.1 | - |
raffinose | pH 5, 75°C | Thermotoga maritima | |
5.32 | - |
raffinose | pH 7, 98°C | Thermotoga neapolitana | |
15.11 | - |
melibiose | pH 7, 98°C | Thermotoga neapolitana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
1 * 61000, SDS-PAGE | Thermotoga neapolitana |
62000 | - |
2 * 62000, SDS-PAGE | Thermotoga maritima |
125000 | - |
gel filtration | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
DSMZ3109 | - |
Thermotoga maritima DSM 3109 | - |
DSMZ3109 | - |
Thermotoga neapolitana | - |
DSMZ5068 | - |
Thermotoga neapolitana DSM Z5068 | - |
DSMZ5068 | - |
Purification (Comment) | Organism |
---|---|
- |
Thermotoga neapolitana |
- |
Thermotoga maritima |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
224 | - |
activity with p-nitrophenyl alpha-D-galactopyranoside | Thermotoga neapolitana |
225 | - |
activity with p-nitrophenyl alpha-D-galactopyranoside | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
melibiose + H2O | - |
Thermotoga neapolitana | D-galactose + alpha-D-glucose | - |
? | |
melibiose + H2O | - |
Thermotoga maritima | D-galactose + alpha-D-glucose | - |
? | |
melibiose + H2O | - |
Thermotoga maritima DSM 3109 | D-galactose + alpha-D-glucose | - |
? | |
melibiose + H2O | - |
Thermotoga neapolitana DSM Z5068 | D-galactose + alpha-D-glucose | - |
? | |
additional information | slight preference for branched galactosyl residues over straight chain residues | Thermotoga neapolitana | ? | - |
? | |
additional information | slight preference for branched galactosyl residues over straight chain residues | Thermotoga neapolitana DSM Z5068 | ? | - |
? | |
o-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermotoga maritima | o-nitrophenol + alpha-D-galactose | - |
? | |
o-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermotoga maritima DSM 3109 | o-nitrophenol + alpha-D-galactose | - |
? | |
p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermotoga neapolitana | p-nitrophenol + alpha-D-galactose | - |
? | |
p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermotoga maritima | p-nitrophenol + alpha-D-galactose | - |
? | |
p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermotoga maritima DSM 3109 | p-nitrophenol + alpha-D-galactose | - |
? | |
p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermotoga neapolitana DSM Z5068 | p-nitrophenol + alpha-D-galactose | - |
? | |
raffinose + H2O | - |
Thermotoga neapolitana | sucrose + D-galactose | - |
? | |
raffinose + H2O | - |
Thermotoga neapolitana DSM Z5068 | sucrose + D-galactose | - |
? | |
raffinose + H2O | - |
Thermotoga maritima | sucrose + alpha-D-galactose | - |
? | |
raffinose + H2O | - |
Thermotoga maritima DSM 3109 | sucrose + alpha-D-galactose | - |
? | |
stachyose + 2 H2O | - |
Thermotoga neapolitana | 2 alpha-D-galactose + sucrose | - |
? | |
stachyose + 2 H2O | 20fold less active than with raffinose | Thermotoga maritima | 2 alpha-D-galactose + sucrose | - |
? | |
stachyose + 2 H2O | 20fold less active than with raffinose | Thermotoga maritima DSM 3109 | 2 alpha-D-galactose + sucrose | - |
? | |
stachyose + 2 H2O | - |
Thermotoga neapolitana DSM Z5068 | 2 alpha-D-galactose + sucrose | - |
? | |
verbascose + H2O | - |
Thermotoga neapolitana | alpha-D-galactose + alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside | - |
? | |
verbascose + H2O | 20fold less active than with raffinose | Thermotoga maritima | alpha-D-galactose + alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 62000, SDS-PAGE | Thermotoga maritima |
monomer | 1 * 61000, SDS-PAGE | Thermotoga neapolitana |
Synonyms | Comment | Organism |
---|---|---|
TmGalA | - |
Thermotoga maritima |
TnGalA | - |
Thermotoga neapolitana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | 95 | - |
Thermotoga maritima |
99 | - |
in 50 mM sodium phosphate buffer, pH 7 | Thermotoga neapolitana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | 95 | more than 50% of maximal activity at 75°C and at 95°C, at pH 5 | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
pH 7, 20 mM Tris-HCl buffer, 1 mg/ml protein concentration, 50% loss of activity after 7 days | Thermotoga maritima |
80 | - |
pH 7, 20 mM Tris-HCl buffer, 1 mg/ml protein concentration, 50% loss of activity after 48 h | Thermotoga maritima |
85 | - |
pH 7, 20 mM Tris-HCl buffer, 1 mg/ml protein concentration, 50% loss of activity after 6 h | Thermotoga maritima |
90 | - |
pH 7, 20 mM Tris-HCl buffer, 1 mg/ml protein concentration, 50% loss of activity after 70 min | Thermotoga maritima |
100 | - |
half-life: 2.5 min | Thermotoga neapolitana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
109 | - |
raffinose | pH 5, 75°C | Thermotoga maritima | |
157.3 | - |
raffinose | pH 7, 98°C | Thermotoga neapolitana | |
176 | - |
p-nitrophenyl alpha-D-galactopyranoside | pH 5, 75°C | Thermotoga maritima | |
234.2 | - |
melibiose | pH 7, 98°C | Thermotoga neapolitana | |
302.9 | - |
p-nitrophenyl alpha-D-galactopyranoside | pH 7, 98°C | Thermotoga neapolitana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | - |
Thermotoga maritima |
7 | - |
at 90°C in 50 mM sodium phosphate buffer | Thermotoga neapolitana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | more than 50% of maximal activity at pH 4.5 and pH 7.0, at 75°C | Thermotoga maritima |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | 75°C, 50 mM citrate-phosphate buffer, less than 40% loss of activity after 5 h | Thermotoga maritima |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermotoga neapolitana | calculation | - |
4.9 |