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Literature summary for 3.2.1.22 extracted from

  • Miller, E.S.; Parker, K.N.; Liebl, W.; Lam, D.; Callem, W.; Snead, M.A.; mathur, E.J.; Short, J.M.; Kelly, R.M.
    alpha-D-Galactosidases from Thermotoga species (2001), Methods Enzymol., 330, 246-260.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermotoga neapolitana
expression in Escherichia coli Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
CuCl2 1 mM, 50% inhibition Thermotoga maritima
FeCl2 1 mM, 50% inhibition Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.075
-
p-nitrophenyl alpha-D-galactopyranoside pH 5, 75°C Thermotoga maritima
0.74
-
p-nitrophenyl alpha-D-galactopyranoside pH 7, 98°C Thermotoga neapolitana
2.1
-
raffinose pH 5, 75°C Thermotoga maritima
5.32
-
raffinose pH 7, 98°C Thermotoga neapolitana
15.11
-
melibiose pH 7, 98°C Thermotoga neapolitana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
1 * 61000, SDS-PAGE Thermotoga neapolitana
62000
-
2 * 62000, SDS-PAGE Thermotoga maritima
125000
-
gel filtration Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
DSMZ3109
-
Thermotoga maritima DSM 3109
-
DSMZ3109
-
Thermotoga neapolitana
-
DSMZ5068
-
Thermotoga neapolitana DSM Z5068
-
DSMZ5068
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermotoga neapolitana
-
Thermotoga maritima

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
224
-
activity with p-nitrophenyl alpha-D-galactopyranoside Thermotoga neapolitana
225
-
activity with p-nitrophenyl alpha-D-galactopyranoside Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
melibiose + H2O
-
Thermotoga neapolitana D-galactose + alpha-D-glucose
-
?
melibiose + H2O
-
Thermotoga maritima D-galactose + alpha-D-glucose
-
?
melibiose + H2O
-
Thermotoga maritima DSM 3109 D-galactose + alpha-D-glucose
-
?
melibiose + H2O
-
Thermotoga neapolitana DSM Z5068 D-galactose + alpha-D-glucose
-
?
additional information slight preference for branched galactosyl residues over straight chain residues Thermotoga neapolitana ?
-
?
additional information slight preference for branched galactosyl residues over straight chain residues Thermotoga neapolitana DSM Z5068 ?
-
?
o-nitrophenyl alpha-D-galactopyranoside + H2O
-
Thermotoga maritima o-nitrophenol + alpha-D-galactose
-
?
o-nitrophenyl alpha-D-galactopyranoside + H2O
-
Thermotoga maritima DSM 3109 o-nitrophenol + alpha-D-galactose
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Thermotoga neapolitana p-nitrophenol + alpha-D-galactose
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Thermotoga maritima p-nitrophenol + alpha-D-galactose
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Thermotoga maritima DSM 3109 p-nitrophenol + alpha-D-galactose
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Thermotoga neapolitana DSM Z5068 p-nitrophenol + alpha-D-galactose
-
?
raffinose + H2O
-
Thermotoga neapolitana sucrose + D-galactose
-
?
raffinose + H2O
-
Thermotoga neapolitana DSM Z5068 sucrose + D-galactose
-
?
raffinose + H2O
-
Thermotoga maritima sucrose + alpha-D-galactose
-
?
raffinose + H2O
-
Thermotoga maritima DSM 3109 sucrose + alpha-D-galactose
-
?
stachyose + 2 H2O
-
Thermotoga neapolitana 2 alpha-D-galactose + sucrose
-
?
stachyose + 2 H2O 20fold less active than with raffinose Thermotoga maritima 2 alpha-D-galactose + sucrose
-
?
stachyose + 2 H2O 20fold less active than with raffinose Thermotoga maritima DSM 3109 2 alpha-D-galactose + sucrose
-
?
stachyose + 2 H2O
-
Thermotoga neapolitana DSM Z5068 2 alpha-D-galactose + sucrose
-
?
verbascose + H2O
-
Thermotoga neapolitana alpha-D-galactose + alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
-
?
verbascose + H2O 20fold less active than with raffinose Thermotoga maritima alpha-D-galactose + alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
-
?

Subunits

Subunits Comment Organism
dimer 2 * 62000, SDS-PAGE Thermotoga maritima
monomer 1 * 61000, SDS-PAGE Thermotoga neapolitana

Synonyms

Synonyms Comment Organism
TmGalA
-
Thermotoga maritima
TnGalA
-
Thermotoga neapolitana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90 95
-
Thermotoga maritima
99
-
in 50 mM sodium phosphate buffer, pH 7 Thermotoga neapolitana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
75 95 more than 50% of maximal activity at 75°C and at 95°C, at pH 5 Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75
-
pH 7, 20 mM Tris-HCl buffer, 1 mg/ml protein concentration, 50% loss of activity after 7 days Thermotoga maritima
80
-
pH 7, 20 mM Tris-HCl buffer, 1 mg/ml protein concentration, 50% loss of activity after 48 h Thermotoga maritima
85
-
pH 7, 20 mM Tris-HCl buffer, 1 mg/ml protein concentration, 50% loss of activity after 6 h Thermotoga maritima
90
-
pH 7, 20 mM Tris-HCl buffer, 1 mg/ml protein concentration, 50% loss of activity after 70 min Thermotoga maritima
100
-
half-life: 2.5 min Thermotoga neapolitana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
109
-
raffinose pH 5, 75°C Thermotoga maritima
157.3
-
raffinose pH 7, 98°C Thermotoga neapolitana
176
-
p-nitrophenyl alpha-D-galactopyranoside pH 5, 75°C Thermotoga maritima
234.2
-
melibiose pH 7, 98°C Thermotoga neapolitana
302.9
-
p-nitrophenyl alpha-D-galactopyranoside pH 7, 98°C Thermotoga neapolitana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5
-
Thermotoga maritima
7
-
at 90°C in 50 mM sodium phosphate buffer Thermotoga neapolitana

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7 more than 50% of maximal activity at pH 4.5 and pH 7.0, at 75°C Thermotoga maritima

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 75°C, 50 mM citrate-phosphate buffer, less than 40% loss of activity after 5 h Thermotoga maritima

pI Value

Organism Comment pI Value Maximum pI Value
Thermotoga neapolitana calculation
-
4.9