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Literature summary for 3.2.1.22 extracted from

  • Ademark, P.; Larsson, M.; Tjerneld, F.; Stalbrand, H.
    Multiple alpha-galactosidases from Aspergillus niger: purification, characterization and substrate specificities (2001), Enzyme Microb. Technol., 29, 441-448.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
galactose powerful inhibitor of alpha-Gal II, alpha-Gal III and alpha-Gal IV. 30% inhibition at 0.5 mM, nearly 100% inhibition at 40 mM Aspergillus niger
mannose 70% inhibition of alpha-Gal I at 40 mM Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
p-nitrophenyl alpha-D-galactopyranoside pH 4.5, 50°C, isoenzyme alpha-Gal II Aspergillus niger
0.24
-
p-nitrophenyl alpha-D-galactopyranoside pH 4.5, 50°C, isoenzyme alpha-Gal IV Aspergillus niger
0.27
-
p-nitrophenyl alpha-D-galactopyranoside pH 4.5, 50°C, isoenzyme alpha-Gal III Aspergillus niger
0.39
-
melibiose pH 4.5, 50°C, isoenzyme alpha-Gal II Aspergillus niger
1
-
melibiose pH 4.5, 50°C, isoenzyme alpha-Gal I Aspergillus niger
1.4
-
p-nitrophenyl alpha-D-galactopyranoside pH 4.5, 50°C, isoenzyme alpha-Gal I Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
2 * 64000, alpha-Gal I, alpha-Gal II and alpha-Gal III, SDS-PAGE Aspergillus niger
117000
-
alpha-Gal I, alpha-Gal II and alpha-Gal III, gel filtration Aspergillus niger
350000
-
alpha-Gal I, gel filtration Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
multiple isoforms: alpha-Gal I, alpha-Gal II, alpha-Gal III, alpha-Gal IV Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.8
-
isoenzyme alpha-Gal III Aspergillus niger
11.52
-
isoenzyme alpha-Gal IV Aspergillus niger
21.95
-
isoenzyme alpha-Gal II Aspergillus niger
1080
-
isoenzyme alpha-Gal I Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
melibiose + H2O
-
Aspergillus niger D-galactose + D-glucose
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Aspergillus niger p-nitrophenol + alpha-D-galactose
-
?
stachyose + 2 H2O high activity with alpha-Gal I, low activity with alpha-Gal II Aspergillus niger 2 alpha-D-galactose + sucrose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 64000, alpha-Gal I, alpha-Gal II and alpha-Gal III, SDS-PAGE Aspergillus niger
tetramer 4 * 117000, SDS-PAGE Aspergillus niger

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus niger isoelectric focusing, isoenzyme alpha-Gal I
-
4.2
Aspergillus niger isoelectric focusing, isoenzyme alpha-Gal II
-
4.5
Aspergillus niger isoelectric focusing, isoenzyme alpha-Gal III
-
4.9
Aspergillus niger isoelectric focusing, isoenzyme alpha-Gal IV
-
4.9