KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
4-methylumbelliferyl alpha-D-galactoside | 50°C, pH 4.5 | Thermomyces lanuginosus | |
2.4 | - |
melibiose | 50°C, pH 4.5 | Thermomyces lanuginosus | |
11.3 | - |
raffinose | 50°C, pH 4.5 | Thermomyces lanuginosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermomyces lanuginosus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
x * 57000, SDS-PAGE | Thermomyces lanuginosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomyces lanuginosus | - |
IMI 158749 | - |
Thermomyces lanuginosus IMI 158749 | - |
IMI 158749 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Thermomyces lanuginosus |
Purification (Comment) | Organism |
---|---|
- |
Thermomyces lanuginosus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Thermomyces lanuginosus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
266.6 | - |
- |
Thermomyces lanuginosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl alpha-D-galactoside + H2O | - |
Thermomyces lanuginosus | 4-methylumbelliferone + alpha-D-galactose | - |
? | |
4-methylumbelliferyl alpha-D-galactoside + H2O | - |
Thermomyces lanuginosus IMI 158749 | 4-methylumbelliferone + alpha-D-galactose | - |
? | |
melibiose + H2O | - |
Thermomyces lanuginosus | alpha-D-galactose + D-glucose | - |
? | |
melibiose + H2O | - |
Thermomyces lanuginosus IMI 158749 | alpha-D-galactose + D-glucose | - |
? | |
raffinose + H2O | - |
Thermomyces lanuginosus | sucrose + alpha-D-galactose | - |
? | |
raffinose + H2O | - |
Thermomyces lanuginosus IMI 158749 | sucrose + alpha-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 57000, SDS-PAGE | Thermomyces lanuginosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 70 | - |
Thermomyces lanuginosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 75 | 50°C: about 60% of maximal activity, 75°C: about 50% of maximal activity | Thermomyces lanuginosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
pH 4.5, 50 mM sodium acetate buffer, stable for 6 h | Thermomyces lanuginosus |
65 | - |
pH 4.5, 50 mM sodium acetate buffer, about 30% loss of activity after 2 h, about 50% loss of activitry after 3 h, about 90% loss of activity after 6 h | Thermomyces lanuginosus |
70 | - |
pH 4.5, 50 mM sodium acetate buffer, about 75% loss of activity after 30 min | Thermomyces lanuginosus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
melibiose | 50°C, pH 4.5 | Thermomyces lanuginosus | |
32.4 | - |
raffinose | 50°C, pH 4.5 | Thermomyces lanuginosus | |
49.8 | - |
4-methylumbelliferyl alpha-D-galactoside | 50°C, pH 4.5 | Thermomyces lanuginosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | - |
Thermomyces lanuginosus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 6 | pH 2.0: about 50% of maximal activity, pH 6.0: about 50% of maximal activity | Thermomyces lanuginosus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 7.5 | 50°C, 5 h, stable | Thermomyces lanuginosus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermomyces lanuginosus | isoelectric focusing, pH-gradient 3-7 | - |
4.9 |