Cloned (Comment) | Organism |
---|---|
mutant enzymes Q279E and R301Q are expressed in Sf9 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Q279E | mutation does not significantly affect the enzymatic activity, but mutant protein level is decreased in the ER of the cell. No difference in the degradative activity between the mutant and the wild-type enzyme with the natural substrate globotriaosylceramide.Mutant enzyme is almost completely inactivated after 30 min at 50°C, while the wild-type enzyme retains 43% of its original activity even after 60 min.The KM-value for 4-methylumbelliferyl alpha-D-galactoside is 1.3fold higher than the wild-type value | Homo sapiens |
R301Q | mutation does not significantly affect the enzymatic activity, but mutant protein level is decreased in the ER of the cell. No difference in the degradative activity between the mutant and the wild-type enzyme with the natural substrate globotriaosylceramide. Mutant enzyme is almost completely inactivated after 30 min at 50°C, while the wild-type enzyme retains 43% of its original activity even after 60 min. The KM-value for 4-methylumbelliferyl alpha-D-galactoside is 1.3fold higher than the wild-type value | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
4-methylumbelliferyl alpha-D-galactoside | wild-type enzyme | Homo sapiens | |
2.8 | - |
4-methylumbelliferyl alpha-D-galactoside | mutant enzyme Q279E and R301Q | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
globotriaosylceramide + H2O | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl alpha-D-galactoside + H2O | - |
Homo sapiens | 4-methylumbelliferone + D-galactose | - |
? | |
globotriaosylceramide + H2O | - |
Homo sapiens | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
mutant enzymes Q279E and R301Q are almost completely inactivated after 30 min at 50°C, while the wild-type enzyme retains 43% of its original activity even after 60 min | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.6 | - |
wild-type and mutant enzymes Q279E and R301Q | Homo sapiens |