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Literature summary for 3.2.1.22 extracted from

  • Ishiguro, M.; Kaneko, S.; Kuno, A.; Koyama, Y.; Yoshida, S.; Park, G.G.; Sakakibara, Y.; Kusakabe, I.; Kobayashi, H.
    Purification and characterization of the recombinant Thermus sp. strain T2 alpha-galactosidase expressed in Escherichia coli (2001), Appl. Environ. Microbiol., 67, 1601-1606.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.22

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermus sp. T2

Protein Variants

Protein Variants Comment Organism
C159A mutant enzyme shows the same specific activity against p-nitrophenyl alpha-D-galactopyranoside as the wild-type enzyme. 1 mM PCMB has no effect in activity of the mutant enzyme, wild-type enzyme is completely inhibited Thermus sp. T2

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, about 80% inhibition Thermus sp. T2
Hg2+ 1 mM, more than 95% inhibition Thermus sp. T2
PCMB 1 mM, 30°C, 30 min, complete inhibition of wild-type enzyme, no inhibition of mutant enzyme C159A Thermus sp. T2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.7
-
 p-nitrophenyl alpha-D-galactopyranoside pH 6.0, 70°C Thermus sp. T2

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53514
-
 8 * 53514, calculation from nucleotide sequence Thermus sp. T2
400000
-
 above, gel filtration Thermus sp. T2

Organism

Organism UniProt Comment Textmining
Thermus sp. T2 Q9WXC1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus sp. T2

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
247
-
-
 Thermus sp. T2

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-[4-O-(6-O-alpha-D-galactopyranosyl-beta-D-mannopyranosyl)-beta-D-mannpyranosyl]-beta-D-mannose + H2O
-
 Thermus sp. T2 alpha-D-galactopyranose + 4-O-[4-O-beta-D-mannopyranosyl-beta-D-mannpyranosyl]-beta-D-mannose
-
 ?
melibiose + H2O
-
 Thermus sp. T2 alpha-D-galactose + D-glucose
-
 ?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Thermus sp. T2 p-nitrophenol + alpha-D-galactose
-
 ?
raffinose + H2O
-
 Thermus sp. T2 sucrose + alpha-D-galactose
-
 ?
stachyose + 2 H2O
-
 Thermus sp. T2 2 alpha-D-galactose + sucrose
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 53514, calculation from nucleotide sequence Thermus sp. T2

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
 reaction with p-nitrophenyl alpha-D-galactopyranoside Thermus sp. T2

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 1 h, stable up to Thermus sp. T2
70
-
 1 h, 50% loss of activity Thermus sp. T2

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 13 40°C, 1 h, stable Thermus sp. T2