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Literature summary for 3.2.1.22 extracted from

  • McCleary, B.V.
    alpha-D-Galactosidase from lucerne and guar seed (1988), Methods Enzymol., 160, 627-632.
No PubMed abstract available
Search for more literature for 3.2.1.22

Organism

Organism UniProt Comment Textmining
Cyamopsis tetragonoloba
-
-
-
Medicago sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Medicago sativa
-
 Cyamopsis tetragonoloba

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon alpha-galactosidase I Cyamopsis tetragonoloba
-
endosperm alpha-galactosidase II Cyamopsis tetragonoloba
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
52.1
-
 alpha-galactosidase II, p-nitrophenyl-alpha-D-galactopyranose as subtrate Cyamopsis tetragonoloba
77.13
-
 alpha-galactosidase II Medicago sativa
81.54
-
 alpha-galactosidase I Medicago sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl-alpha-D-galactoside + H2O
-
 Cyamopsis tetragonoloba p-nitrophenol + alpha-D-galactose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Cyamopsis tetragonoloba

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 alpha-galactosidase II, unstable above Medicago sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5 alpha-galactosidase II Cyamopsis tetragonoloba
4.5 5.5 alpha-galactosidase II Medicago sativa