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Literature summary for 3.2.1.212 extracted from

  • Silchenko, A.S.; Ustyuzhanina, N.E.; Kusaykin, M.I.; Krylov, V.B.; Shashkov, A.S.; Dmitrenok, A.S.; Usoltseva, R.V.; Zueva, A.O.; Nifantiev, N.E.; Zvyagintseva, T.N.
    Expression and biochemical characterization and substrate specificity of the fucoidanase from Formosa algae (2017), Glycobiology, 27, 254-263 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Ba2+ activates Formosa algae
Ca2+ activates Formosa algae
Co2+ activates Formosa algae
Mg2+ activates Formosa algae
Mn2+ activates Formosa algae

Application

Application Comment Organism
synthesis the enzyme can be used for the modification of natural fucoidans to obtain more regular and easier characterized derivatives useful for research and practical applications Formosa algae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Formosa algae

Inhibitors

Inhibitors Comment Organism Structure
Al3+
-
Formosa algae
Cu2+
-
Formosa algae
EDTA the addition of EDTA accompanied by desalting of a medium completely inactivates the enzyme Formosa algae
Fe3+
-
Formosa algae
Sn2+
-
Formosa algae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
101200
-
calculated from sequence Formosa algae

Organism

Organism UniProt Comment Textmining
Formosa algae WP_057784219.1
-
-
Formosa algae KMM 3553T WP_057784219.1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Formosa algae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fucoidan + H2O fucoidanase FFA2 catalyzes the cleavage of (1->4)-alpha-glycosidic bonds in the fucoidan from Fucus evanescens within a structural fragment [(->3)-alpha-L-Fucp2S-(1->4)-alpha-L-Fucp2S-(1->)]n but not in a fragment [(->3)-alpha-L-Fucp2S,4S-(1->4)-alpha-L-Fucp2S-(1->)]n. A broad number of sulfated oligosaccharides is formed after several minutes of the reaction start. Tetra- and disaccharides are formed after 15 min and 3 h of the reaction, respectively Formosa algae sulfated alpha-L-fucooligosaccharides
-
?
fucoidan + H2O fucoidanase FFA2 catalyzes the cleavage of (1->4)-alpha-glycosidic bonds in the fucoidan from Fucus evanescens within a structural fragment [(->3)-alpha-L-Fucp2S-(1->4)-alpha-L-Fucp2S-(1->)]n but not in a fragment [(->3)-alpha-L-Fucp2S,4S-(1->4)-alpha-L-Fucp2S-(1->)]n. A broad number of sulfated oligosaccharides is formed after several minutes of the reaction start. Tetra- and disaccharides are formed after 15 min and 3 h of the reaction, respectively Formosa algae KMM 3553T sulfated alpha-L-fucooligosaccharides
-
?
additional information unsulfated or persulfated oligosaccharides are not cleaved. Tetrasaccharide and synthetic sulfated tetrasaccharide with bonds sequence (1->3)-(1->4)-(1->3) reacts much slower than the tetrasaccharide bearing bonds sequence (1->4)-(1->3)-(1->4) Formosa algae ?
-
?
additional information unsulfated or persulfated oligosaccharides are not cleaved. Tetrasaccharide and synthetic sulfated tetrasaccharide with bonds sequence (1->3)-(1->4)-(1->3) reacts much slower than the tetrasaccharide bearing bonds sequence (1->4)-(1->3)-(1->4) Formosa algae KMM 3553T ?
-
?
propyl alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-) + H2O
-
Formosa algae propyl alpha-L-Fucp(2OSO3-) + alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)
-
?
propyl alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-) + H2O
-
Formosa algae KMM 3553T propyl alpha-L-Fucp(2OSO3-) + alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)
-
?
propyl alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-) + H2O efficiently cleaved to form a complex mixture of mono- and oligosaccharides Formosa algae ?
-
?
propyl alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-) + H2O efficiently cleaved to form a complex mixture of mono- and oligosaccharides Formosa algae KMM 3553T ?
-
?

Synonyms

Synonyms Comment Organism
FFA2
-
Formosa algae
poly[(1->4)-alpha-L-fucoside-2-sulfate] glycanohydrolase
-
Formosa algae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 37
-
Formosa algae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 9
-
Formosa algae