Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activates | Formosa algae | |
Ca2+ | activates | Formosa algae | |
Co2+ | activates | Formosa algae | |
Mg2+ | activates | Formosa algae | |
Mn2+ | activates | Formosa algae |
Application | Comment | Organism |
---|---|---|
synthesis | the enzyme can be used for the modification of natural fucoidans to obtain more regular and easier characterized derivatives useful for research and practical applications | Formosa algae |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Formosa algae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | - |
Formosa algae | |
Cu2+ | - |
Formosa algae | |
EDTA | the addition of EDTA accompanied by desalting of a medium completely inactivates the enzyme | Formosa algae | |
Fe3+ | - |
Formosa algae | |
Sn2+ | - |
Formosa algae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
101200 | - |
calculated from sequence | Formosa algae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Formosa algae | WP_057784219.1 | - |
- |
Formosa algae KMM 3553T | WP_057784219.1 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Formosa algae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
fucoidan + H2O | fucoidanase FFA2 catalyzes the cleavage of (1->4)-alpha-glycosidic bonds in the fucoidan from Fucus evanescens within a structural fragment [(->3)-alpha-L-Fucp2S-(1->4)-alpha-L-Fucp2S-(1->)]n but not in a fragment [(->3)-alpha-L-Fucp2S,4S-(1->4)-alpha-L-Fucp2S-(1->)]n. A broad number of sulfated oligosaccharides is formed after several minutes of the reaction start. Tetra- and disaccharides are formed after 15 min and 3 h of the reaction, respectively | Formosa algae | sulfated alpha-L-fucooligosaccharides | - |
? | |
fucoidan + H2O | fucoidanase FFA2 catalyzes the cleavage of (1->4)-alpha-glycosidic bonds in the fucoidan from Fucus evanescens within a structural fragment [(->3)-alpha-L-Fucp2S-(1->4)-alpha-L-Fucp2S-(1->)]n but not in a fragment [(->3)-alpha-L-Fucp2S,4S-(1->4)-alpha-L-Fucp2S-(1->)]n. A broad number of sulfated oligosaccharides is formed after several minutes of the reaction start. Tetra- and disaccharides are formed after 15 min and 3 h of the reaction, respectively | Formosa algae KMM 3553T | sulfated alpha-L-fucooligosaccharides | - |
? | |
additional information | unsulfated or persulfated oligosaccharides are not cleaved. Tetrasaccharide and synthetic sulfated tetrasaccharide with bonds sequence (1->3)-(1->4)-(1->3) reacts much slower than the tetrasaccharide bearing bonds sequence (1->4)-(1->3)-(1->4) | Formosa algae | ? | - |
? | |
additional information | unsulfated or persulfated oligosaccharides are not cleaved. Tetrasaccharide and synthetic sulfated tetrasaccharide with bonds sequence (1->3)-(1->4)-(1->3) reacts much slower than the tetrasaccharide bearing bonds sequence (1->4)-(1->3)-(1->4) | Formosa algae KMM 3553T | ? | - |
? | |
propyl alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-) + H2O | - |
Formosa algae | propyl alpha-L-Fucp(2OSO3-) + alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) | - |
? | |
propyl alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-) + H2O | - |
Formosa algae KMM 3553T | propyl alpha-L-Fucp(2OSO3-) + alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-) | - |
? | |
propyl alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-) + H2O | efficiently cleaved to form a complex mixture of mono- and oligosaccharides | Formosa algae | ? | - |
? | |
propyl alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-)-(1->3)-alpha-L-Fucp(2OSO3-)-(1->4)-alpha-L-Fucp(2OSO3-) + H2O | efficiently cleaved to form a complex mixture of mono- and oligosaccharides | Formosa algae KMM 3553T | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FFA2 | - |
Formosa algae |
poly[(1->4)-alpha-L-fucoside-2-sulfate] glycanohydrolase | - |
Formosa algae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | - |
Formosa algae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | - |
Formosa algae |