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Literature summary for 3.2.1.21 extracted from

  • Hannemann, L.; Lucaciu, C.R.; Sharma, S.; Rattei, T.; Mayer, K.F.X.; Gierl, A.; Frey, M.
    A promiscuous beta-glucosidase is involved in benzoxazinoid deglycosylation in Lamium galeobdolon (2018), Phytochemistry, 156, 224-233 .
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.9
-
Dhurrin isoform GLU1, at pH 5.5 and 30°C Lamium galeobdolon
2.5
-
2,4-dihydroxy-1,4-benzoxazin-3-one glucoside isoform GLU1, at pH 5.5 and 30°C Lamium galeobdolon
4.1
-
oleuropein isoform GLU1, at pH 5.5 and 30°C Lamium galeobdolon

Organism

Organism UniProt Comment Textmining
Lamium galeobdolon A0A3G1VCA8
-
-
Lamium galeobdolon A0A3G1VCA9
-
-
Lamium galeobdolon A0A3G1VCB1
-
-
Lamium galeobdolon A0A3G1VCB6
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Lamium galeobdolon
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
isoform GLU2, with phloridzin as substrate, at pH 5.5 and 30°C Lamium galeobdolon
0.005
-
isoform GLU4, with 2,4-dihydroxy-1,4-benzoxazin-3-one glucoside as substrate, at pH 5.5 and 30°C Lamium galeobdolon
0.013
-
isoform GLU1, with trans-zeatin-O-glucoside as substrate, at pH 5.5 and 30°C Lamium galeobdolon
0.053
-
isoform GLU2, with daidzin as substrate, at pH 5.5 and 30°C Lamium galeobdolon
0.065
-
isoform GLU1, with phloridzin as substrate, at pH 5.5 and 30°C Lamium galeobdolon
0.071
-
isoform GLU1, with geniposide as substrate, at pH 5.5 and 30°C Lamium galeobdolon
0.077
-
isoform GLU4, with oleuropein as substrate, at pH 5.5 and 30°C Lamium galeobdolon
0.078
-
isoform GLU4, with genistin as substrate, at pH 5.5 and 30°C Lamium galeobdolon
0.086
-
isoform GLU2, with 2,4-dihydroxy-1,4-benzoxazin-3-one glucoside as substrate, at pH 5.5 and 30°C Lamium galeobdolon
0.104
-
isoform GLU2, with 4-nitrophenyl beta-D-fucopyranoside as substrate, at pH 5.5 and 30°C Lamium galeobdolon
0.104
-
isoform GLU4, with 4-nitrophenyl beta-D-fucopyranoside as substrate, at pH 5.5 and 30°C Lamium galeobdolon
0.149
-
isoform GLU4, with daidzin as substrate, at pH 5.5 and 30°C Lamium galeobdolon
0.283
-
isoform GLU1, with 4-nitrophenyl beta-D-cellobioside as substrate, at pH 5.5 and 30°C Lamium galeobdolon
0.291
-
isoform GLU2, with 4-nitrophenyl beta-D-cellobioside as substrate, at pH 5.5 and 30°C Lamium galeobdolon
0.291
-
isoform GLU4, with 4-nitrophenyl beta-D-cellobioside as substrate, at pH 5.5 and 30°C Lamium galeobdolon
0.34
-
isoform GLU4, with dhurrin as substrate, at pH 5.5 and 30°C Lamium galeobdolon
0.472
-
isoform GLU2, with 4-nitrophenyl beta-D-glucopyranoside as substrate, at pH 5.5 and 30°C Lamium galeobdolon
0.58
-
isoform GLU2, with oleuropein as substrate, at pH 5.5 and 30°C Lamium galeobdolon
0.949
-
isoform GLU2, with dhurrin as substrate, at pH 5.5 and 30°C Lamium galeobdolon
1.933
-
isoform GLU1, with genistin as substrate, at pH 5.5 and 30°C Lamium galeobdolon
2.436
-
isoform GLU1, with daidzin as substrate, at pH 5.5 and 30°C Lamium galeobdolon
5.814
-
isoform GLU4, with 4-nitrophenyl beta-D-glucopyranoside as substrate, at pH 5.5 and 30°C Lamium galeobdolon
7.139
-
isoform GLU1, with dhurrin as substrate, at pH 5.5 and 30°C Lamium galeobdolon
7.967
-
isoform GLU1, with oleuropein as substrate, at pH 5.5 and 30°C Lamium galeobdolon
8.66
-
isoform GLU1, with 4-nitrophenyl beta-D-glucopyranoside as substrate, at pH 5.5 and 30°C Lamium galeobdolon
34.083
-
isoform GLU1, with 2,4-dihydroxy-1,4-benzoxazin-3-one glucoside as substrate, at pH 5.5 and 30°C Lamium galeobdolon

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dihydroxy-1,4-benzoxazin-3-one glucoside + H2O
-
Lamium galeobdolon ?
-
?
2,4-dihydroxy-1,4-benzoxazin-3-one glucoside + H2O best substrate for isoform GLU1 Lamium galeobdolon ?
-
?
4-nitrophenyl beta-D-cellobioside + H2O about 10% activity compared to 4-nitrophenyl beta-D-glucopyranoside Lamium galeobdolon 4-nitrophenol + D-cellobiose
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
-
Lamium galeobdolon 4-nitrophenol + D-fucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Lamium galeobdolon 4-nitrophenol + beta-D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O best substrate for isoform GLU4 Lamium galeobdolon 4-nitrophenol + beta-D-glucopyranose
-
?
daidzin + H2O
-
Lamium galeobdolon daidzein + D-glucose
-
?
dhurrin + H2O
-
Lamium galeobdolon (S)-4-hydroxymandelonitrile + D-glucose
-
?
dhurrin + H2O best substrate for isoform GLU2 Lamium galeobdolon (S)-4-hydroxymandelonitrile + D-glucose
-
?
geniposide + H2O
-
Lamium galeobdolon geniposide aglycone + D-glucose
-
?
genistin + H2O
-
Lamium galeobdolon genistein + D-glucose
-
?
additional information 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl beta-D-mannopyranoside, harpagide, arbutin, and trans-zeatin-O-glucoside are no substrates Lamium galeobdolon ?
-
?
additional information 4-nitrophenyl beta-D-mannopyranoside, harpagide, arbutin, geniposide, genistin, and trans-zeatin-O-glucoside are no substrates Lamium galeobdolon ?
-
?
additional information 4-nitrophenyl beta-D-mannopyranoside, harpagide, geniposide, arbutin, and trans-zeatin-O-glucoside are no substrates Lamium galeobdolon ?
-
?
oleuropein + H2O
-
Lamium galeobdolon oleuropein aglycone + D-glucose
-
?
phloridzin + H2O
-
Lamium galeobdolon phloridzin aglycone + D-glucose
-
?

Synonyms

Synonyms Comment Organism
BGLU
-
Lamium galeobdolon
Glu1 isoform Lamium galeobdolon
Glu2 isoform Lamium galeobdolon
GLU3 isoform Lamium galeobdolon
GLU4 isoform Lamium galeobdolon

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
104.4
-
oleuropein isoform GLU1, at pH 5.5 and 30°C Lamium galeobdolon
112.5
-
Dhurrin isoform GLU1, at pH 5.5 and 30°C Lamium galeobdolon
211.1
-
2,4-dihydroxy-1,4-benzoxazin-3-one glucoside isoform GLU1, at pH 5.5 and 30°C Lamium galeobdolon

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
25.5
-
oleuropein isoform GLU1, at pH 5.5 and 30°C Lamium galeobdolon
59
-
Dhurrin isoform GLU1, at pH 5.5 and 30°C Lamium galeobdolon
81.6
-
2,4-dihydroxy-1,4-benzoxazin-3-one glucoside isoform GLU1, at pH 5.5 and 30°C Lamium galeobdolon