Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DMSO | 10% (v/v), native enzyme is activated to 102.3% of control, recombinant enzyme is activated to 106.4% of control, beta-glucosidase activity | Putranjiva roxburghii | |
EDTA | 1 mM, native enzyme is activated to 110.7% of control, recombinant enzyme is activated to 108.2% of control, beta-glucosidase activity | Putranjiva roxburghii | |
SDS | 1 mg/ml, native enzyme is activated to 118.3% of control, recombinant enzyme is activated to 115.6% of control, beta-glucosidase activity | Putranjiva roxburghii | |
Triton X-100 | 10 mg/ml, native enzyme is activated to 113.4% of control, recombinant enzyme is activated to 111.7% of control, beta-glucosidase activity | Putranjiva roxburghii |
Application | Comment | Organism |
---|---|---|
industry | potentially an important industrial enzyme due to the broad specificity, catalytic efficiency and thermostability | Putranjiva roxburghii |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Putranjiva roxburghii |
Protein Variants | Comment | Organism |
---|---|---|
E173N | mutant enzyme shows no activity | Putranjiva roxburghii |
E389N | mutant enzyme shows no activity | Putranjiva roxburghii |
E446N | beta-fucosidase activity is 26.49% compared to wild-type activity, beta-glucosidase activity is 21.37% compared to wild-type activity, beta-galactosidase activity is 15.7% compared to wild-type activity | Putranjiva roxburghii |
N172A | beta-fucosidase activity is 18.38% compared to wild-type activity, beta-glucosidase activity is 17.65% compared to wild-type activity, beta-galactosidase activity is 13.4% compared to wild-type activity | Putranjiva roxburghii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1 mM, 9% loss of activity of native enzyme, 10% loss of activity of recombinant enzyme, beta-glucosidase activity | Putranjiva roxburghii | |
Cr3+ | 1 mM, 29% loss of activity of native enzyme, 30% loss of activity of recombinant enzyme, beta-glucosidase activity | Putranjiva roxburghii | |
delta-gluconolactone | 5 mM, 85% loss of activity of native enzyme, 86% loss of activity of recombinant enzyme, beta-glucosidase activity | Putranjiva roxburghii | |
DTT | 1 mM, 4% loss of activity of native enzyme, 5% loss of activity of recombinant enzyme, beta-glucosidase activity | Putranjiva roxburghii | |
Guanidine HCl | 10 mM, 8% loss of activity of native enzyme, 10% loss of activity of recombinant enzyme, beta-glucosidase activity | Putranjiva roxburghii | |
Hg2+ | 1 mM, 55% loss of activity of native enzyme, 58% loss of activity of recombinant enzyme, beta-glucosidase activity | Putranjiva roxburghii | |
Mg2+ | 1 mM, 7% loss of activity, native and recombinant enzyme, beta-glucosidase activity | Putranjiva roxburghii | |
additional information | significant resistant to D-glucose concentration up to 500 mM | Putranjiva roxburghii | |
N-bromosuccinamide | 2 mM, 92% loss of activity of native enzyme, 94% loss of activity of recombinant enzyme, beta-glucosidase activity | Putranjiva roxburghii | |
Ni2+ | 1 mM, 16% loss of activity of native enzyme, 18% loss of activity of recombinant enzyme, beta-glucosidase activity | Putranjiva roxburghii | |
Zn2+ | 1 mM, 47% loss of activity of native enzyme, 52% loss of activity of recombinant enzyme, beta-glucosidase activity | Putranjiva roxburghii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii | |
0.51 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
0.53 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii | |
0.58 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
0.64 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii | |
0.68 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
0.81 | - |
cellobiose | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii | |
0.87 | - |
cellobiose | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM, native enzyme is activated to 111.6% of control, recombinant enzyme is activated to 109.1% of control, beta-glucosidase activity | Putranjiva roxburghii | |
Fe2+ | 1 mM, native enzyme is activated to 130.0% of control, recombinant enzyme is activated to 127.3% of control, beta-glucosidase activity | Putranjiva roxburghii | |
Mn2+ | 1 mM, native enzyme is activated to 102.3% of control, recombinant enzyme is activated to 106.4% of control, beta-glucosidase activity | Putranjiva roxburghii | |
Pb2+ | 1 mM, native enzyme is activated to 109.3% of control, recombinant enzyme is activated to 110.6% of control, beta-glucosidase activity | Putranjiva roxburghii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Putranjiva roxburghii | A0A023MIF8 | - |
- |
Purification (Comment) | Organism |
---|---|
a single step concanvalin A affinity chromatography for native PRGH1 improves the yield and reduced the purification time | Putranjiva roxburghii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-fucopyranoside + H2O | relative activity of 125% as compared to 4-nitrophenyl beta-D-glucopyranoside. Maximum hydrolytic activity for beta-D-fucopyranoside followed by 4-nitrophenyl beta-D-glucopyranoside and 4-nitrophenyl beta-D-galactopyranoside | Putranjiva roxburghii | 4-nitrophenol + beta-D-fucopyranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | maximum hydrolytic activity for beta-D-fucopyranoside followed by 4-nitrophenyl beta-D-glucopyranoside and 4-nitrophenyl beta-D-galactopyranoside | Putranjiva roxburghii | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | maximum hydrolytic activity for beta-D-fucopyranoside followed by 4-nitrophenyl beta-D-glucopyranoside and 4-nitrophenyl beta-D-galactopyranoside | Putranjiva roxburghii | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
cellobiose + H2O | - |
Putranjiva roxburghii | 2 beta-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PRGH1 | - |
Putranjiva roxburghii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
native and recombinant enzyme, beta-glucosidase activity | Putranjiva roxburghii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 65 | native and recombinant enzymes are stable | Putranjiva roxburghii |
70 | - |
1 h, the native enzyme retains 67% of its activity while the recombinant enzyme preserves 35% | Putranjiva roxburghii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.82 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
6.95 | - |
cellobiose | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
7.39 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii | |
8.12 | - |
cellobiose | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii | |
10.39 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
12 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii | |
12.71 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
14.77 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.6 | - |
recombinant enzyme, glucosidase activity | Putranjiva roxburghii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | native and recombinant enzyme are stable over a broad pH range of 4.0-8.0 with maximum retention of their catalytic activity. However, activities decline quickly below pH 4.0 | Putranjiva roxburghii |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme may be involved in plant defence | Putranjiva roxburghii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7.99 | - |
cellobiose | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
8.57 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
10.1 | - |
cellobiose | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii | |
11.5 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii | |
14.9 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
17.9 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 4.6, 37°C, recombinant enzyme | Putranjiva roxburghii | |
22.6 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii | |
32.1 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 4.6, 37°C, native enzyme | Putranjiva roxburghii |