Activating Compound | Comment | Organism | Structure |
---|---|---|---|
4-hydroxybenzoic acid | 109.7% activity at 2.5 mM with cellobioside as substrate | Thermothelomyces thermophilus | |
p-coumaric acid | 118% activity at 2.5 mM with cellobioside as substrate | Thermothelomyces thermophilus | |
syringic acid | 111.4% activity at 2.5 mM with cellobioside as substrate | Thermothelomyces thermophilus | |
vanillic acid | 125.1% activity at 2.5 mM with cellobioside as substrate | Thermothelomyces thermophilus | |
Vanillin | 118% activity at 2.5 mM with cellobioside as substrate | Thermothelomyces thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxybenzoic acid | 97.5% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate | Thermothelomyces thermophilus | |
acetone | 68.3% and 62% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
Al3+ | 83.5% and 43.7% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
Ba2+ | 76.7% residual activity at 2.5 mM with cellobiose as substrate | Thermothelomyces thermophilus | |
Cu2+ | complete inhibition at 2.5 mM | Thermothelomyces thermophilus | |
D-glucose | competitive inhibition | Thermothelomyces thermophilus | |
dithiothreitol | 82.5% residual activity and complete inhibition at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
DMSO | 92.9% and 57% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
ethanol | 70.6% and 55.6% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
Fe2+ | complete inhibition at 2.5 mM | Thermothelomyces thermophilus | |
ferulic acid | 97.4% and 99.1% residual activity at 0.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
gallic acid | 91.1% and 75.3% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
Isopropanol | 66.5-70.1% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
K+ | 76.2% and 81.9% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
Mg2+ | 62.7% residual activity at 2.5 mM with cellobiose as substrate | Thermothelomyces thermophilus | |
Mn2+ | 49.6% and 67.1% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
Na+ | 70.6% and 57.1% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
p-coumaric acid | 70.5% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate | Thermothelomyces thermophilus | |
PEG 8000 | 67.6% and 58.5% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
polyvinyl alcohol | 71.2% and 56.8% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
SDS | 82.4% and 60.6% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
syringaldehyde | 92.8% and 96.4% residual activity at 0.25 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
syringic acid | 95.2% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate | Thermothelomyces thermophilus | |
Tannic acid | complete inhibition at 0.0625 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate | Thermothelomyces thermophilus | |
Triton X-100 | 57.5% and 45% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus | |
vanillic acid | 87.6% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate | Thermothelomyces thermophilus | |
Vanillin | 94% residual activity at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside as substrate | Thermothelomyces thermophilus | |
Zn2+ | 63% residual activity and complete inhibition at 2.5 mM with 4-nitrophenyl beta-D-glucopyranoside and cellobioside as substrate, respectively | Thermothelomyces thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
4-nitrophenyl beta-D-glucopyranoside | at pH 5.0 and 60°C | Thermothelomyces thermophilus | |
8.5 | - |
cellobiose | at pH 5.0 and 50°C | Thermothelomyces thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Li+ | 102.6-128.4% activity at 2.5 mM | Thermothelomyces thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermothelomyces thermophilus | - |
- |
- |
Thermothelomyces thermophilus M.7.7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose resin column chromatography and Sephacrly S-100 gel filtration | Thermothelomyces thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermothelomyces thermophilus | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermothelomyces thermophilus M.7.7 | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
cellobiose + H2O | - |
Thermothelomyces thermophilus | 2 beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Thermothelomyces thermophilus M.7.7 | 2 beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Thermothelomyces thermophilus |
Synonyms | Comment | Organism |
---|---|---|
BGL50 | - |
Thermothelomyces thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
with cellobiose as substrate | Thermothelomyces thermophilus |
60 | - |
with 4-nitrophenyl beta-D-glucopyranoside as substrate | Thermothelomyces thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the enzyme is very stable at 60°C displaying a half-life of 855.6 min but it is easily denatured above this temperature | Thermothelomyces thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
with cellobiose as substrate | Thermothelomyces thermophilus |
5 | - |
with 4-nitrophenyl beta-D-glucopyranoside as substrate | Thermothelomyces thermophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
D-glucose | at pH 5.0 and 60°C | Thermothelomyces thermophilus |