Literature summary for 3.2.1.21 extracted from

Haq, I.; Khan, M.; Muneer, B.; Hussain, Z.; Afzal, S.; Majeed, S.; Rashid, N.; Javed, M.; Ahmad, I.
Cloning, characterization and molecular docking of a highly thermostable beta-1,4-glucosidase from Thermotoga petrophila (2012), Biotechnol. Lett., 34, 1703-1709 .

Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli strain BL21 Codon-Plus	Thermotoga petrophila

Inhibitors

Inhibitors	Comment	Organism	Structure
1-propanol	40% (v/v), activity is reduced to 2%	Thermotoga petrophila
ethanol	40% (v/v), activity is reduced to 2%	Thermotoga petrophila
methanol	40% (v/v), activity is reduced to 2%	Thermotoga petrophila
additional information	not inhibited by glucose up to 5% (w/v)	Thermotoga petrophila

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.8	-	4-nitrophenyl beta-D-glucopyranoside	pH 7.0, 90°C	Thermotoga petrophila
4.3	-	4-nitrophenyl beta-D-cellobioside	pH 7.0, 90°C	Thermotoga petrophila
25	-	4-nitrophenyl beta-D-xylopyranoside	pH 7.0, 90°C	Thermotoga petrophila

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cu2+	1 mM, reduces activity to 20%	Thermotoga petrophila
Hg2+	1 mM, reduces activity to 90%	Thermotoga petrophila

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
51500	-	SDS-PAGE	Thermotoga petrophila

Organism

Organism	UniProt	Comment	Textmining
Thermotoga petrophila	A5IL97	-	-
Thermotoga petrophila DSM 13995	A5IL97	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermotoga petrophila

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-cellobioside + H2O	-	Thermotoga petrophila	4-nitrophenol + D-cellobiose	-	?
4-nitrophenyl beta-D-cellobioside + H2O	-	Thermotoga petrophila DSM 13995	4-nitrophenol + D-cellobiose	-	?
4-nitrophenyl beta-D-fucopyranoside + H2O	-	Thermotoga petrophila	4-nitrophenol + D-fucopyranose	-	?
4-nitrophenyl beta-D-fucopyranoside + H2O	-	Thermotoga petrophila DSM 13995	4-nitrophenol + D-fucopyranose	-	?
4-nitrophenyl beta-D-galactopyranoside + H2O	-	Thermotoga petrophila	4-nitrophenol + D-galactopyranose	-	?
4-nitrophenyl beta-D-galactopyranoside + H2O	-	Thermotoga petrophila DSM 13995	4-nitrophenol + D-galactopyranose	-	?
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Thermotoga petrophila	4-nitrophenol + D-glucopyranose	-	?
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Thermotoga petrophila DSM 13995	4-nitrophenol + D-glucopyranose	-	?
4-nitrophenyl beta-D-xylopyranoside + H2O	-	Thermotoga petrophila	4-nitrophenol + D-xylose	-	?
4-nitrophenyl beta-D-xylopyranoside + H2O	-	Thermotoga petrophila DSM 13995	4-nitrophenol + D-xylose	-	?

Synonyms

Synonyms	Comment	Organism
BglA	-	Thermotoga petrophila

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
80	90	-	Thermotoga petrophila

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	100	30°C: about 40% of maximal activity, 100°C: about 30% of maximal activity	Thermotoga petrophila

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
5300	-	4-nitrophenyl beta-D-xylopyranoside	pH 7.0, 90°C	Thermotoga petrophila
17500	-	4-nitrophenyl beta-D-cellobioside	pH 7.0, 90°C	Thermotoga petrophila
87400	-	4-nitrophenyl beta-D-glucopyranoside	pH 7.0, 90°C	Thermotoga petrophila

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	8	-	Thermotoga petrophila

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	9	pH 4.0: about 40% of maximal activity, pH 9.0: about 90% of maximal activity	Thermotoga petrophila

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
210	-	4-nitrophenyl beta-D-xylopyranoside	pH 7.0, 90°C	Thermotoga petrophila
4100	-	4-nitrophenyl beta-D-cellobioside	pH 7.0, 90°C	Thermotoga petrophila
30800	-	4-nitrophenyl beta-D-glucopyranoside	pH 7.0, 90°C	Thermotoga petrophila

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Release 2023.1 (January 2023)