Literature summary for 3.2.1.21 extracted from

Harnpicharnchai, P.; Champreda, V.; Sornlake, W.; Eurwilaichitr, L.
A thermotolerant beta-glucosidase isolated from an endophytic fungi, Periconia sp., with a possible use for biomass conversion to sugars (2009), Protein Expr. Purif., 67, 1-9. .

Search for more literature for 3.2.1.21

Activating Compound

Activating Compound	Comment	Organism	Structure
ethanol	10%, 127% of initial activity, 50%, 0.6% of initial activity	Periconia sp. BCC 2871
glycerol	10%, 121% of initial activity	Periconia sp. BCC 2871
methanol	10%, 121% of initial activity, 50%, 1.3% of initial activity	Periconia sp. BCC 2871
propan-2-ol	10%, 107% of initial activity, 50%, 0.2% of initial activity	Periconia sp. BCC 2871

Application

Application	Comment	Organism
degradation	addition of beta-glucosidase to the rice straw hydrolysis reaction containing a commercial cellulase results in increase of reducing sugars being released	Periconia sp. BCC 2871

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Periconia sp. BCC 2871

Inhibitors

Inhibitors	Comment	Organism	Structure
Cu2+	1 mM, 5% residual activity	Periconia sp. BCC 2871
D-glucose	-	Periconia sp. BCC 2871
EDTA	1 mM, 74% residual activity	Periconia sp. BCC 2871
Fe2+	1 mM, 30% residual activity	Periconia sp. BCC 2871
SDS	1%, 8% residual activity	Periconia sp. BCC 2871
Zn2+	1 mM, 51% residual activity	Periconia sp. BCC 2871

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.04	-	4-methylumbelliferyl beta-D-cellobioside	pH 6.0, 70°C	Periconia sp. BCC 2871
0.19	-	4-nitrophenyl beta-D-glucopyranoside	pH 6.0, 70°C	Periconia sp. BCC 2871
0.5	-	cellobiose	pH 6.0, 70°C	Periconia sp. BCC 2871

Organic Solvent Stability

Organic Solvent	Comment	Organism
Ethanol	10%, 127% of initial activity, 50%, 0.6% of initial activity	Periconia sp. BCC 2871
Glycerol	10%, 121% of initial activity	Periconia sp. BCC 2871
isopropanol	10%, 107% of initial activity, 50%, 0.2% of initial activity	Periconia sp. BCC 2871
Methanol	10%, 121% of initial activity, 50%, 1.3% of initial activity	Periconia sp. BCC 2871

Organism

Organism	UniProt	Comment	Textmining
Periconia sp. BCC 2871	A9UIG0	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	sequence contains at least 16 potentiyl N-glycosylation sites. Treatment with PNGase F leads to a decrease in molecular mass	Periconia sp. BCC 2871

Storage Stability

Storage Stability	Organism
4°C, MOPS buffer, pH 6, stable for at least 6 months	Periconia sp. BCC 2871

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl beta-D-cellobioside + H2O	-	Periconia sp. BCC 2871	4-methylumbelliferone + cellobiose	-	?
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Periconia sp. BCC 2871	4-nitrophenol + D-glucose	-	?
carboxymethylcellulose + H2O	-	Periconia sp. BCC 2871	D-glucose + ?	-	?
cellobiose + H2O	-	Periconia sp. BCC 2871	2 D-glucose	-	?
rice straw + H2O	-	Periconia sp. BCC 2871	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 113000, SDS-PAGE	Periconia sp. BCC 2871

Synonyms

Synonyms	Comment	Organism
BGL1	-	Periconia sp. BCC 2871

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
70	-	-	Periconia sp. BCC 2871

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
65	75	more than 90% of maximum activity	Periconia sp. BCC 2871

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	-	1.5 h, 60% residual activity	Periconia sp. BCC 2871

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	6	-	Periconia sp. BCC 2871

pH Range

pH Minimum	pH Maximum	Comment	Organism
4	7	more than 50% of maximum activity	Periconia sp. BCC 2871

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
8	-	2 h, no loss of activity	Periconia sp. BCC 2871

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
20	-	D-glucose	pH 6.0, 70°C	Periconia sp. BCC 2871

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Release 2023.1 (January 2023)