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Literature summary for 3.2.1.21 extracted from

  • Pei, X.; Zhao, J.; Cai, P.; Sun, W.; Ren, J.; Wu, Q.; Zhang, S.; Tian, C.
    Heterologous expression of a GH3 beta-glucosidase from Neurospora crassa in Pichia pastoris with high purity and its application in the hydrolysis of soybean isoflavone glycosides (2016), Protein Expr. Purif., 119, 75-84 .
    View publication on PubMed
Search for more literature for 3.2.1.21

Activating Compound

Activating Compound Comment Organism Structure
DMSO 10% v/v, 106% of initial activity, 20% v/v, 112.6% of initial activity Neurospora crassa
ethanol 10% v/v, 180% of initial activity, 20% v/v, 70% of initial activity Neurospora crassa

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Neurospora crassa

Inhibitors

Inhibitors Comment Organism Structure
ethanol 10% v/v, 180% of initial activity, 20% v/v, 70% of initial activity Neurospora crassa
SDS 1 mM, 66.5% residual activity Neurospora crassa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 50°C Neurospora crassa

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO 10% v/v, 106% of initial activity, 20% v/v, 112.6% of initial activity Neurospora crassa
Ethanol 10% v/v, 180% of initial activity, 20% v/v, 70% of initial activity Neurospora crassa

Organism

Organism UniProt Comment Textmining
Neurospora crassa Q7S3R5
-
-
Neurospora crassa DSM 1257 Q7S3R5
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein sequence contains two potential N-glycosylation sites and sixteen O-glycosylation sites Neurospora crassa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
47.6
-
 substrate gentobiose, pH 5.0, 50°C Neurospora crassa
75
-
 substrate cellobiose, pH 5.0, 50°C Neurospora crassa
143
-
 substrate 4-nitrophenyl beta-D-glucopyranoside, pH 5.0, 50°C Neurospora crassa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O
-
 Neurospora crassa 4-nitrophenol + cellobiose
-
 ?
4-nitrophenyl beta-D-cellobioside + H2O
-
 Neurospora crassa DSM 1257 4-nitrophenol + cellobiose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Neurospora crassa 4-nitrophenol + D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Neurospora crassa DSM 1257 4-nitrophenol + D-glucose
-
 ?
cellobiose + H2O
-
 Neurospora crassa 2 D-glucose
-
 ?
cellobiose + H2O
-
 Neurospora crassa DSM 1257 2 D-glucose
-
 ?
cellotetraose + 3 H2O
-
 Neurospora crassa 4 D-glucose
-
 ?
cellotetraose + 3 H2O
-
 Neurospora crassa DSM 1257 4 D-glucose
-
 ?
cellotriose + 2 H2O
-
 Neurospora crassa 3 D-glucose
-
 ?
cellotriose + 2 H2O
-
 Neurospora crassa DSM 1257 3 D-glucose
-
 ?
daidzin + H2O
-
 Neurospora crassa daidzein + D-glucose
-
 ?
genistein 7-beta-D-glucopyranoside + H2O
-
 Neurospora crassa genistein + D-glucose
-
 ?
gentobiose + H2O
-
 Neurospora crassa 2 D-glucose
-
 ?
glycitein 7-O-beta-D-glucopyranoside + H2O
-
 Neurospora crassa glycitein + D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 75900, calculated Neurospora crassa

Synonyms

Synonyms Comment Organism
Bgl2
-
 Neurospora crassa
Gh3-4
-
 Neurospora crassa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Neurospora crassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Neurospora crassa

pI Value

Organism Comment pI Value Maximum pI Value
Neurospora crassa calculated
-
 6.2