Cloned (Comment) | Organism |
---|---|
exprression in Escherichia coli | Thermotoga petrophila |
Crystallization (Comment) | Organism |
---|---|
molecular modeling of structure | Thermotoga petrophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | - |
Thermotoga petrophila | |
D-xylose | - |
Thermotoga petrophila | |
additional information | not inhibitory: EDTA (10 mM), 1% of Urea, 2-mercaptoethanol, Triton X-100, and Tween-80 | Thermotoga petrophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 85°C | Thermotoga petrophila | |
1.11 | - |
4-nitrophenyl beta-D-cellobioside | pH 5.0, 85°C | Thermotoga petrophila | |
1.34 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 5.0, 85°C | Thermotoga petrophila | |
2.35 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 85°C | Thermotoga petrophila | |
2.95 | - |
4-nitrophenyl alpha-D-fucopyranoside | pH 5.0, 85°C | Thermotoga petrophila | |
7.35 | - |
cellobiose | pH 5.0, 85°C | Thermotoga petrophila |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, 175% of initial activity | Thermotoga petrophila | |
Co2+ | 10 mM, 110% of initial activity | Thermotoga petrophila | |
Mn2+ | 10 mM, 115% of initial activity | Thermotoga petrophila | |
Zn2+ | 10 mM, 120% of initial activity | Thermotoga petrophila |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 20% v/v, not inhibitory, 50% v/v, 50% loss of activity | Thermotoga petrophila |
acetonitrile | 20% v/v, not inhibitory, 50% v/v, 54% loss of activity | Thermotoga petrophila |
dimethylformamide | 20% v/v, not inhibitory, 50% v/v, 48% loss of activity | Thermotoga petrophila |
DMSO | 20% v/v, not inhibitory, 50% v/v, 50% loss of activity | Thermotoga petrophila |
Ethanol | 10%, 140% of initial activity | Thermotoga petrophila |
Ethyl acetate | 20% v/v, not inhibitory, 50% v/v, 56% loss of activity | Thermotoga petrophila |
isopropanol | 10%, 155% of initial activity | Thermotoga petrophila |
Methanol | 10%, 148% of initial activity | Thermotoga petrophila |
n-Butanol | 10%, 151% of initial activity | Thermotoga petrophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga petrophila | D2C716 | - |
- |
Thermotoga petrophila DSM 13996 | D2C716 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
130000 | - |
pH 5.0, 85°C | Thermotoga petrophila |
Storage Stability | Organism |
---|---|
4°C, purified, crude supernatant, and heat-treated enzyme remaine stable for 1 year | Thermotoga petrophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-fucopyranoside + H2O | - |
Thermotoga petrophila | 4-nitrophenol + D-fucopyranose | - |
? | |
4-nitrophenyl beta-D-cellobioside + H2O | - |
Thermotoga petrophila | 4-nitrophenol + cellobiose | - |
? | |
4-nitrophenyl beta-D-cellobioside + H2O | - |
Thermotoga petrophila DSM 13996 | 4-nitrophenol + cellobiose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Thermotoga petrophila | 4-nitrophenol + D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Thermotoga petrophila DSM 13996 | 4-nitrophenol + D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga petrophila | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga petrophila DSM 13996 | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermotoga petrophila | 4-nitrophenol + D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Thermotoga petrophila DSM 13996 | 4-nitrophenol + D-xylopyranose | - |
? | |
cellobiose + H2O | - |
Thermotoga petrophila | 2 D-glucose | - |
? | |
cellobiose + H2O | - |
Thermotoga petrophila DSM 13996 | 2 D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 81000, SDS-PAGE | Thermotoga petrophila |
Synonyms | Comment | Organism |
---|---|---|
BglB | - |
Thermotoga petrophila |
Tnap_0656 | - |
Thermotoga petrophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Thermotoga petrophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 95 | more than 75% of maximum activity | Thermotoga petrophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1469.8 | - |
cellobiose | pH 5.0, 85°C | Thermotoga petrophila | |
78809.5 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 85°C | Thermotoga petrophila | |
83730.2 | - |
4-nitrophenyl beta-D-cellobioside | pH 5.0, 85°C | Thermotoga petrophila | |
114048 | - |
4-nitrophenyl alpha-D-fucopyranoside | pH 5.0, 85°C | Thermotoga petrophila | |
232540 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 5.0, 85°C | Thermotoga petrophila | |
1214290 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 85°C | Thermotoga petrophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Thermotoga petrophila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | - |
Thermotoga petrophila |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
150 | - |
D-glucose | pH 5.0, 85°C | Thermotoga petrophila | |
200 | - |
D-xylose | pH 5.0, 85°C | Thermotoga petrophila |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
200 | - |
cellobiose | pH 5.0, 85°C | Thermotoga petrophila | |
33536 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 5.0, 85°C | Thermotoga petrophila | |
38660 | - |
4-nitrophenyl alpha-D-fucopyranoside | pH 5.0, 85°C | Thermotoga petrophila | |
75433 | - |
4-nitrophenyl beta-D-cellobioside | pH 5.0, 85°C | Thermotoga petrophila | |
173537 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 5.0, 85°C | Thermotoga petrophila | |
2698410 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 85°C | Thermotoga petrophila |