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Literature summary for 3.2.1.21 extracted from

  • Akram, F.; Haq, I.; Mukhtar, H.
    Gene cloning, characterization and thermodynamic analysis of a novel multidomain hyperthermophilic GH family 3 beta-glucosidase (TnBglB) from Thermotoga naphthophila RKU-10T (2018), Process Biochem., 66, 70-81 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
exprression in Escherichia coli Thermotoga petrophila

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling of structure Thermotoga petrophila

Inhibitors

Inhibitors Comment Organism Structure
D-glucose
-
Thermotoga petrophila
D-xylose
-
Thermotoga petrophila
additional information not inhibitory: EDTA (10 mM), 1% of Urea, 2-mercaptoethanol, Triton X-100, and Tween-80 Thermotoga petrophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
4-nitrophenyl beta-D-glucopyranoside pH 5.0, 85°C Thermotoga petrophila
1.11
-
4-nitrophenyl beta-D-cellobioside pH 5.0, 85°C Thermotoga petrophila
1.34
-
4-nitrophenyl beta-D-xylopyranoside pH 5.0, 85°C Thermotoga petrophila
2.35
-
4-nitrophenyl beta-D-galactopyranoside pH 5.0, 85°C Thermotoga petrophila
2.95
-
4-nitrophenyl alpha-D-fucopyranoside pH 5.0, 85°C Thermotoga petrophila
7.35
-
cellobiose pH 5.0, 85°C Thermotoga petrophila

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10 mM, 175% of initial activity Thermotoga petrophila
Co2+ 10 mM, 110% of initial activity Thermotoga petrophila
Mn2+ 10 mM, 115% of initial activity Thermotoga petrophila
Zn2+ 10 mM, 120% of initial activity Thermotoga petrophila

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 20% v/v, not inhibitory, 50% v/v, 50% loss of activity Thermotoga petrophila
acetonitrile 20% v/v, not inhibitory, 50% v/v, 54% loss of activity Thermotoga petrophila
dimethylformamide 20% v/v, not inhibitory, 50% v/v, 48% loss of activity Thermotoga petrophila
DMSO 20% v/v, not inhibitory, 50% v/v, 50% loss of activity Thermotoga petrophila
Ethanol 10%, 140% of initial activity Thermotoga petrophila
Ethyl acetate 20% v/v, not inhibitory, 50% v/v, 56% loss of activity Thermotoga petrophila
isopropanol 10%, 155% of initial activity Thermotoga petrophila
Methanol 10%, 148% of initial activity Thermotoga petrophila
n-Butanol 10%, 151% of initial activity Thermotoga petrophila

Organism

Organism UniProt Comment Textmining
Thermotoga petrophila D2C716
-
-
Thermotoga petrophila DSM 13996 D2C716
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
130000
-
pH 5.0, 85°C Thermotoga petrophila

Storage Stability

Storage Stability Organism
4°C, purified, crude supernatant, and heat-treated enzyme remaine stable for 1 year Thermotoga petrophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-fucopyranoside + H2O
-
Thermotoga petrophila 4-nitrophenol + D-fucopyranose
-
?
4-nitrophenyl beta-D-cellobioside + H2O
-
Thermotoga petrophila 4-nitrophenol + cellobiose
-
?
4-nitrophenyl beta-D-cellobioside + H2O
-
Thermotoga petrophila DSM 13996 4-nitrophenol + cellobiose
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
Thermotoga petrophila 4-nitrophenol + D-galactopyranose
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
Thermotoga petrophila DSM 13996 4-nitrophenol + D-galactopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Thermotoga petrophila 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Thermotoga petrophila DSM 13996 4-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Thermotoga petrophila 4-nitrophenol + D-xylopyranose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Thermotoga petrophila DSM 13996 4-nitrophenol + D-xylopyranose
-
?
cellobiose + H2O
-
Thermotoga petrophila 2 D-glucose
-
?
cellobiose + H2O
-
Thermotoga petrophila DSM 13996 2 D-glucose
-
?

Subunits

Subunits Comment Organism
? x * 81000, SDS-PAGE Thermotoga petrophila

Synonyms

Synonyms Comment Organism
BglB
-
Thermotoga petrophila
Tnap_0656
-
Thermotoga petrophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Thermotoga petrophila

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 95 more than 75% of maximum activity Thermotoga petrophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1469.8
-
cellobiose pH 5.0, 85°C Thermotoga petrophila
78809.5
-
4-nitrophenyl beta-D-galactopyranoside pH 5.0, 85°C Thermotoga petrophila
83730.2
-
4-nitrophenyl beta-D-cellobioside pH 5.0, 85°C Thermotoga petrophila
114048
-
4-nitrophenyl alpha-D-fucopyranoside pH 5.0, 85°C Thermotoga petrophila
232540
-
4-nitrophenyl beta-D-xylopyranoside pH 5.0, 85°C Thermotoga petrophila
1214290
-
4-nitrophenyl beta-D-glucopyranoside pH 5.0, 85°C Thermotoga petrophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Thermotoga petrophila

pH Range

pH Minimum pH Maximum Comment Organism
4 6
-
Thermotoga petrophila

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
150
-
D-glucose pH 5.0, 85°C Thermotoga petrophila
200
-
D-xylose pH 5.0, 85°C Thermotoga petrophila

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
200
-
cellobiose pH 5.0, 85°C Thermotoga petrophila
33536
-
4-nitrophenyl beta-D-galactopyranoside pH 5.0, 85°C Thermotoga petrophila
38660
-
4-nitrophenyl alpha-D-fucopyranoside pH 5.0, 85°C Thermotoga petrophila
75433
-
4-nitrophenyl beta-D-cellobioside pH 5.0, 85°C Thermotoga petrophila
173537
-
4-nitrophenyl beta-D-xylopyranoside pH 5.0, 85°C Thermotoga petrophila
2698410
-
4-nitrophenyl beta-D-glucopyranoside pH 5.0, 85°C Thermotoga petrophila