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Literature summary for 3.2.1.21 extracted from

  • Liu, Y.; Li, R.; Wang, J.; Zhang, X.; Jia, R.; Gao, Y.; Peng, H.
    Increased enzymatic hydrolysis of sugarcane bagasse by a novel glucose- and xylose-stimulated beta-glucosidase from Anoxybacillus flavithermus subsp. yunnanensis E13T (2017), BMC Biochem., 18, 004 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.21

Application

Application Comment Organism
degradation the supplementation of BglP significantly enhances the glucose yield from sugarcane bagasse, especially in the presence of high concentrations of glucose or xylose Anoxybacillus flavithermus subsp. yunnanensis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Anoxybacillus flavithermus subsp. yunnanensis

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ 5 mM, 77% residual activity Anoxybacillus flavithermus subsp. yunnanensis
SDS 5 mM, 4% residual activity Anoxybacillus flavithermus subsp. yunnanensis
Triton X-100 1%, 91% residual activity Anoxybacillus flavithermus subsp. yunnanensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
 4-nitrophenyl beta-D-glucopyranoside pH 7.0, 60°C Anoxybacillus flavithermus subsp. yunnanensis
12.3
-
 cellobiose pH 7.0, 60°C Anoxybacillus flavithermus subsp. yunnanensis

Organism

Organism UniProt Comment Textmining
Anoxybacillus flavithermus subsp. yunnanensis S5U834
-
-
Anoxybacillus flavithermus subsp. yunnanensis E13T S5U834
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-glucopyranoside + H2O 51% of the activity with 4-nitrophenyl beta-D-glucopyranoside Anoxybacillus flavithermus subsp. yunnanensis 2-nitrophenol + beta-D-glucose
-
 ?
2-nitrophenyl beta-D-glucopyranoside + H2O 51% of the activity with 4-nitrophenyl beta-D-glucopyranoside Anoxybacillus flavithermus subsp. yunnanensis E13T 2-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Anoxybacillus flavithermus subsp. yunnanensis 4-nitrophenol + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Anoxybacillus flavithermus subsp. yunnanensis E13T 4-nitrophenol + beta-D-glucose
-
 ?
cellobiose + H2O 17.5% of the activity with 4-nitrophenyl beta-D-glucopyranoside Anoxybacillus flavithermus subsp. yunnanensis 2 D-glucose
-
 ?
cellobiose + H2O 17.5% of the activity with 4-nitrophenyl beta-D-glucopyranoside Anoxybacillus flavithermus subsp. yunnanensis E13T 2 D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 52000, SDS-PAGE and calculated Anoxybacillus flavithermus subsp. yunnanensis

Synonyms

Synonyms Comment Organism
BglP
-
 Anoxybacillus flavithermus subsp. yunnanensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Anoxybacillus flavithermus subsp. yunnanensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70
-
 50% of maximum activity Anoxybacillus flavithermus subsp. yunnanensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 hlaf-life 10 h Anoxybacillus flavithermus subsp. yunnanensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Anoxybacillus flavithermus subsp. yunnanensis

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5 more than 80% of maximum activity Anoxybacillus flavithermus subsp. yunnanensis