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Literature summary for 3.2.1.21 extracted from
- Optimization of a heat-tolerant beta-glucosidase production by Bacillus sp. ZJ1308 and its purification and characterization (2016), Biotechnol. Appl. Biochem., 63, 553-563 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | optimized medium composition for beta-glucosidase expression is corn cob (51.8 g/l), beef extract (23.8 g/l), salicin (0.5 g/l), MnSO4·H2O (0.363 g/l), MgSO4·7H2O (0.4 g/l), and NaCl (5 g/l). Under the optimal conditions, the activity of beta-glucosidase is up to 4.71 U/ml | Bacillus sp. ZJ1308 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 5 mM, 19% residual activity | Bacillus sp. ZJ1308 |  |
| Ca2+ | 5 mM, 68% residual activity | Bacillus sp. ZJ1308 | |
| Co2+ | 5 mM, 58% residual activity | Bacillus sp. ZJ1308 | |
| Cu2+ | 5 mM, 48% residual activity | Bacillus sp. ZJ1308 | |
| K+ | 5 mM, 62% residual activity | Bacillus sp. ZJ1308 | |
| Mg2+ | 5 mM, 72% residual activity | Bacillus sp. ZJ1308 | |
| Mn2+ | 5 mM, 83% residual activity | Bacillus sp. ZJ1308 | |
| Ni2+ | 5 mM, 21% residual activity | Bacillus sp. ZJ1308 | |
| Zn2+ | 5 mM, 63% residual activity | Bacillus sp. ZJ1308 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
gel filtration | Bacillus sp. ZJ1308 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus sp. ZJ1308 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus sp. ZJ1308 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 110.5 | - |
pH 7.0, 50°C | Bacillus sp. ZJ1308 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Bacillus sp. ZJ1308 | 4-nitrophenol + beta-D-galactose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Bacillus sp. ZJ1308 | 4-nitrophenol + beta-D-glucose | - |
? | |
| salicin + H2O | - |
Bacillus sp. ZJ1308 | saligenin + beta-D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Bacillus sp. ZJ1308 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
30 min, 56% residual activity | Bacillus sp. ZJ1308 |
| 90 | - |
30 min, 38% residual activity | Bacillus sp. ZJ1308 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Bacillus sp. ZJ1308 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Bacillus sp. ZJ1308 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 700 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 7.0, 50°C | Bacillus sp. ZJ1308 | |
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