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Literature summary for 3.2.1.21 extracted from

  • Hong, J.; Tamaki, H.; Kumagai, H.
    Cloning and functional expression of thermostable beta-glucosidase gene from Thermoascus aurantiacus (2007), Appl. Microbiol. Biotechnol., 73, 1331-1339 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Thermoascus aurantiacus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.24
-
4-nitrophenyl beta-D-glucopyranoside pH 5.0, 40°C Thermoascus aurantiacus
0.37
-
4-nitrophenyl beta-cellobioside pH 5.0, 40°C Thermoascus aurantiacus
1.25
-
cellobiose pH 5.0, 40°C Thermoascus aurantiacus
3.25
-
4-nitrophenyl beta-D-xylopyranoside pH 5.0, 40°C Thermoascus aurantiacus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermoascus aurantiacus
-
-

Organism

Organism UniProt Comment Textmining
Thermoascus aurantiacus Q0ZUL0
-
-
Thermoascus aurantiacus IFO 9748 Q0ZUL0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-cellobioside + 2 H2O
-
Thermoascus aurantiacus 4-nitrophenol + 2 D-glucose
-
?
4-nitrophenyl beta-cellobioside + 2 H2O
-
Thermoascus aurantiacus IFO 9748 4-nitrophenol + 2 D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Thermoascus aurantiacus 4-nitrophenol + D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Thermoascus aurantiacus IFO 9748 4-nitrophenol + D-glucose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Thermoascus aurantiacus 4-nitrophenol + D-xylose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Thermoascus aurantiacus IFO 9748 4-nitrophenol + D-xylose
-
?
cellobiose + H2O
-
Thermoascus aurantiacus 2 D-glucose
-
?
cellobiose + H2O
-
Thermoascus aurantiacus IFO 9748 2 D-glucose
-
?

Synonyms

Synonyms Comment Organism
BGL1
-
Thermoascus aurantiacus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Thermoascus aurantiacus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
1 h, 70% residual activity Thermoascus aurantiacus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Thermoascus aurantiacus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 8
-
Thermoascus aurantiacus