Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | at up to 15% v/v | Pholiota adiposa | |
methanol | at up to 15% v/v | Pholiota adiposa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.6 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.0, 65°C | Pholiota adiposa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Pholiota adiposa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | strong inhibition at 5 mM | Pholiota adiposa | |
Fe2+ | 25% inhibition at 5 mM | Pholiota adiposa | |
Hg2+ | strong inhibition at 5 mM | Pholiota adiposa | |
additional information | Ca2+, Co2+, Mg2+, Mn2+, and Zn2+ have no inhibitory effects at 5 mM. Addition of beta-mercaptoethanol or cysteine does not significantly alter activity | Pholiota adiposa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
gel filtration | Pholiota adiposa |
60000 | - |
1 * 60000, SDS-PAGE | Pholiota adiposa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cellobiose + H2O | Pholiota adiposa | - |
2 beta-D-glucose | - |
? | |
cellobiose + H2O | Pholiota adiposa SKU0714 | - |
2 beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pholiota adiposa | - |
- |
- |
Pholiota adiposa SKU0714 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Pholiota adiposa |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 130.3fold from culture supernatant by ultrafiltration, anion exchange chromatography and gel filtration to homogeneity | Pholiota adiposa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Pholiota adiposa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2088 | - |
purified enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside, pH 5.0, 65°C | Pholiota adiposa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pholiota adiposa | 2-nitrophenol + beta-D-glucose | - |
? | |
2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pholiota adiposa SKU0714 | 2-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-cellobioside + H2O | - |
Pholiota adiposa | 4-nitrophenol + cellobiose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pholiota adiposa | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Pholiota adiposa SKU0714 | 4-nitrophenol + beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Pholiota adiposa | 2 beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Pholiota adiposa SKU0714 | 2 beta-D-glucose | - |
? | |
cellopentaose + H2O | - |
Pholiota adiposa | ? | - |
? | |
cellotetraose + H2O | - |
Pholiota adiposa | ? | - |
? | |
cellotriose + H2O | - |
Pholiota adiposa | ? | - |
? | |
cellotriose + H2O | - |
Pholiota adiposa SKU0714 | ? | - |
? | |
additional information | no activity with caroxymethyl cellulose, xylan, Avicel, and laminarin, poor activity with 4-nitrophenyl-beta-D-galactopyranoside | Pholiota adiposa | ? | - |
? | |
additional information | no activity with caroxymethyl cellulose, xylan, Avicel, and laminarin, poor activity with 4-nitrophenyl-beta-D-galactopyranoside | Pholiota adiposa SKU0714 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, SDS-PAGE | Pholiota adiposa |
Synonyms | Comment | Organism |
---|---|---|
Bgl | - |
Pholiota adiposa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Pholiota adiposa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 70 | 20% of maximal activity at 40°C, 80% at 70°C, profile overview | Pholiota adiposa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3460 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.0, 65°C | Pholiota adiposa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Pholiota adiposa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7.5 | activity range, profile overview | Pholiota adiposa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | - |
purified enzyme, 25°C, 24 h, over 20% activity remaining | Pholiota adiposa |
3 | 6 | purified enzyme, 24 h, over 80% activity remaining | Pholiota adiposa |
7 | - |
purified enzyme, 25°C, 24 h, over 50% activity remaining | Pholiota adiposa |
8 | - |
purified enzyme, 25°C, 24 h, over 20% activity remaining | Pholiota adiposa |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycoside hydrolase family 3, GH3 | Pholiota adiposa |