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Literature summary for 3.2.1.21 extracted from

  • Jagtap, S.; Dhiman, S.; Kim, T.; Li, J.; Chan Kang, Y.; Lee, J.
    Characterization of a beta-1,4-glucosidase from a newly isolated strain of Pholiota adiposa and its application to the hydrolysis of biomass (2013), Biomass Bioenergy, 54, 181-190.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
ethanol at up to 15% v/v Pholiota adiposa
methanol at up to 15% v/v Pholiota adiposa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.6
-
4-nitrophenyl-beta-D-glucopyranoside pH 5.0, 65°C Pholiota adiposa

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Pholiota adiposa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ strong inhibition at 5 mM Pholiota adiposa
Fe2+ 25% inhibition at 5 mM Pholiota adiposa
Hg2+ strong inhibition at 5 mM Pholiota adiposa
additional information Ca2+, Co2+, Mg2+, Mn2+, and Zn2+ have no inhibitory effects at 5 mM. Addition of beta-mercaptoethanol or cysteine does not significantly alter activity Pholiota adiposa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
gel filtration Pholiota adiposa
60000
-
1 * 60000, SDS-PAGE Pholiota adiposa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cellobiose + H2O Pholiota adiposa
-
2 beta-D-glucose
-
?
cellobiose + H2O Pholiota adiposa SKU0714
-
2 beta-D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Pholiota adiposa
-
-
-
Pholiota adiposa SKU0714
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Pholiota adiposa

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 130.3fold from culture supernatant by ultrafiltration, anion exchange chromatography and gel filtration to homogeneity Pholiota adiposa

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Pholiota adiposa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2088
-
purified enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside, pH 5.0, 65°C Pholiota adiposa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-glucopyranoside + H2O
-
Pholiota adiposa 2-nitrophenol + beta-D-glucose
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
-
Pholiota adiposa SKU0714 2-nitrophenol + beta-D-glucose
-
?
4-nitrophenyl beta-cellobioside + H2O
-
Pholiota adiposa 4-nitrophenol + cellobiose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Pholiota adiposa 4-nitrophenol + beta-D-glucose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Pholiota adiposa SKU0714 4-nitrophenol + beta-D-glucose
-
?
cellobiose + H2O
-
Pholiota adiposa 2 beta-D-glucose
-
?
cellobiose + H2O
-
Pholiota adiposa SKU0714 2 beta-D-glucose
-
?
cellopentaose + H2O
-
Pholiota adiposa ?
-
?
cellotetraose + H2O
-
Pholiota adiposa ?
-
?
cellotriose + H2O
-
Pholiota adiposa ?
-
?
cellotriose + H2O
-
Pholiota adiposa SKU0714 ?
-
?
additional information no activity with caroxymethyl cellulose, xylan, Avicel, and laminarin, poor activity with 4-nitrophenyl-beta-D-galactopyranoside Pholiota adiposa ?
-
?
additional information no activity with caroxymethyl cellulose, xylan, Avicel, and laminarin, poor activity with 4-nitrophenyl-beta-D-galactopyranoside Pholiota adiposa SKU0714 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE Pholiota adiposa

Synonyms

Synonyms Comment Organism
Bgl
-
Pholiota adiposa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Pholiota adiposa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 20% of maximal activity at 40°C, 80% at 70°C, profile overview Pholiota adiposa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3460
-
4-nitrophenyl-beta-D-glucopyranoside pH 5.0, 65°C Pholiota adiposa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Pholiota adiposa

pH Range

pH Minimum pH Maximum Comment Organism
3 7.5 activity range, profile overview Pholiota adiposa

pH Stability

pH Stability pH Stability Maximum Comment Organism
2
-
purified enzyme, 25°C, 24 h, over 20% activity remaining Pholiota adiposa
3 6 purified enzyme, 24 h, over 80% activity remaining Pholiota adiposa
7
-
purified enzyme, 25°C, 24 h, over 50% activity remaining Pholiota adiposa
8
-
purified enzyme, 25°C, 24 h, over 20% activity remaining Pholiota adiposa

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycoside hydrolase family 3, GH3 Pholiota adiposa