Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-gluconolactone | competitive inhibition | Galleria mellonella | |
D-glucose | high tolerance towards D-glucose inhibition with 4-nitrophenyl beta-D-glucopyranoside as substrate | Galleria mellonella | |
delta-gluconolactone | - |
Galleria mellonella |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
4-nitrophenyl beta-D-glucopyranoside | in 50 mM sodium acetate, pH 5.5, at 37°C | Galleria mellonella | |
1.9 | - |
2-nitrophenyl beta-D-glucopyranoside | in 50 mM sodium acetate, pH 5.5, at 37°C | Galleria mellonella |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
x * 42000, SDS-PAGE | Galleria mellonella |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Galleria mellonella | - |
greater wax moth | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and Sepharose-4B-L-tyrosine-1-naphthylamine column chromatography | Galleria mellonella |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | - |
Galleria mellonella | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.42 | - |
crude extract, in 50 mM sodium acetate, pH 5.5, at 37°C | Galleria mellonella |
24.36 | - |
after 57.5fold purification, in 50 mM sodium acetate, pH 5.5, at 37°C | Galleria mellonella |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | 35% activity compared to 4-nitrophenyl beta-D-glucopyranoside | Galleria mellonella | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
2-nitrophenyl beta-D-glucopyranoside + H2O | 109% activity compared to 4-nitrophenyl beta-D-glucopyranoside | Galleria mellonella | 2-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl 1-thio-beta-D-glucopyranoside + H2O | 1% activity compared to 4-nitrophenyl beta-D-glucopyranoside | Galleria mellonella | 4-nitrophenol + 1-thio-beta-D-glucopyranoside | - |
? | |
4-nitrophenyl beta-D-fucopyranoside + H2O | 455% activity compared to 4-nitrophenyl beta-D-glucopyranoside | Galleria mellonella | 4-nitrophenol + beta-D-fucopyranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | 41% activity compared to 4-nitrophenyl beta-D-glucopyranoside | Galleria mellonella | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | 100% activity | Galleria mellonella | 4-nitrophenol + D-glucopyranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42000, SDS-PAGE | Galleria mellonella |
Synonyms | Comment | Organism |
---|---|---|
beta-D-glucoside glucohydrolase | - |
Galleria mellonella |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
- |
Galleria mellonella |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | more than 50% activity between 30 and 50°C | Galleria mellonella |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Galleria mellonella |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6.5 | more than 50% activity between pH 3.0 and 6.5 | Galleria mellonella |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
delta-gluconolactone | in 50 mM sodium acetate, pH 5.5, at 37°C | Galleria mellonella |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.27 | - |
in 50 mM sodium acetate, pH 5.5, at 37°C | Galleria mellonella | delta-gluconolactone | |
213.1 | - |
in 50 mM sodium acetate, pH 5.5, at 37°C | Galleria mellonella | D-glucose |