Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.21 extracted from

  • Kara, H.E.; Turan, Y.; Er, A.; Acar, M.; Tuemay, S.; Sinan, S.
    Purification and characterization of beta-glucosidase from greater wax moth Galleria mellonella L. (Lepidoptera: Pyralidae) (2014), Arch. Insect Biochem. Physiol., 86, 209-219.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
beta-gluconolactone competitive inhibition Galleria mellonella
D-glucose high tolerance towards D-glucose inhibition with 4-nitrophenyl beta-D-glucopyranoside as substrate Galleria mellonella
delta-gluconolactone
-
Galleria mellonella

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
4-nitrophenyl beta-D-glucopyranoside in 50 mM sodium acetate, pH 5.5, at 37°C Galleria mellonella
1.9
-
2-nitrophenyl beta-D-glucopyranoside in 50 mM sodium acetate, pH 5.5, at 37°C Galleria mellonella

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
x * 42000, SDS-PAGE Galleria mellonella

Organism

Organism UniProt Comment Textmining
Galleria mellonella
-
greater wax moth
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Sepharose-4B-L-tyrosine-1-naphthylamine column chromatography Galleria mellonella

Source Tissue

Source Tissue Comment Organism Textmining
larva
-
Galleria mellonella
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.42
-
crude extract, in 50 mM sodium acetate, pH 5.5, at 37°C Galleria mellonella
24.36
-
after 57.5fold purification, in 50 mM sodium acetate, pH 5.5, at 37°C Galleria mellonella

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O 35% activity compared to 4-nitrophenyl beta-D-glucopyranoside Galleria mellonella 2-nitrophenol + beta-D-galactopyranose
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O 109% activity compared to 4-nitrophenyl beta-D-glucopyranoside Galleria mellonella 2-nitrophenol + beta-D-glucose
-
?
4-nitrophenyl 1-thio-beta-D-glucopyranoside + H2O 1% activity compared to 4-nitrophenyl beta-D-glucopyranoside Galleria mellonella 4-nitrophenol + 1-thio-beta-D-glucopyranoside
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O 455% activity compared to 4-nitrophenyl beta-D-glucopyranoside Galleria mellonella 4-nitrophenol + beta-D-fucopyranose
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O 41% activity compared to 4-nitrophenyl beta-D-glucopyranoside Galleria mellonella 4-nitrophenol + beta-D-galactopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O 100% activity Galleria mellonella 4-nitrophenol + D-glucopyranose
-
?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Galleria mellonella

Synonyms

Synonyms Comment Organism
beta-D-glucoside glucohydrolase
-
Galleria mellonella

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
-
Galleria mellonella

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 more than 50% activity between 30 and 50°C Galleria mellonella

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Galleria mellonella

pH Range

pH Minimum pH Maximum Comment Organism
3 6.5 more than 50% activity between pH 3.0 and 6.5 Galleria mellonella

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.021
-
delta-gluconolactone in 50 mM sodium acetate, pH 5.5, at 37°C Galleria mellonella

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.27
-
in 50 mM sodium acetate, pH 5.5, at 37°C Galleria mellonella delta-gluconolactone
213.1
-
in 50 mM sodium acetate, pH 5.5, at 37°C Galleria mellonella D-glucose