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Literature summary for 3.2.1.21 extracted from

  • Oh, H.J.; Shin, K.C.; Oh, D.K.
    Production of ginsenosides Rg1 and Rh1 by hydrolyzing the outer glycoside at the C-6 position in protopanaxatriol-type ginsenosides using beta-glucosidase from Pyrococcus furiosus (2013), Biotechnol. Lett., 36, 113-119.
    View publication on PubMed
Search for more literature for 3.2.1.21

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q51723
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.05
-
 pH 5.5, 95°C, substrate: ginsenoside Rg2 Pyrococcus furiosus
0.06
-
 pH 5.5, 95°C, substrate: ginsenoside R2 Pyrococcus furiosus
0.23
-
 pH 5.5, 95°C, substrate: ginsenoside Re Pyrococcus furiosus
0.6
-
 pH 5.5, 95°C, substrate: 4-nitrophenyl-alpha-L-rhamnopyranoside Pyrococcus furiosus
0.69
-
 pH 5.5, 95°C, substrate: ginsenoside R1 Pyrococcus furiosus
6.8
-
 pH 5.5, 95°C, substrate: ginsenoside Rf Pyrococcus furiosus
12.5
-
 pH 5.5, 95°C, substrate: 2-nitrophenyl-beta-D-xylopyranoside Pyrococcus furiosus
117
-
 pH 5.5, 95°C, substrate: 4-nitrophenyl-beta-D-xylopyranoside Pyrococcus furiosus
828
-
 pH 5.5, 95°C, substrate: 2-nitrophenyl-beta-D-glucopyranoside Pyrococcus furiosus
1300
-
 pH 5.5, 95°C, substrate: 4-nitrophenyl-beta-D-glucopyranoside Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 2-nitrophenol + D-glucopyranose
-
 ?
2-nitrophenyl beta-D-xylopyranoside + H2O
-
 Pyrococcus furiosus 2-nitrophenol + beta-D-xylopyranose
-
 ?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + alpha-L-rhamnopyranose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Pyrococcus furiosus 4-nitrophenol + beta-D-xylopyranose
-
 ?
ginsenoside R1 + H2O
-
 Pyrococcus furiosus ginsenoside Rg1 + D-xylose ginsenoside Rg1 i.e. 6alpha,20-bis(beta-D-glucopyranosyl)oxy-dammar-24-en-3beta,12beta-diol ?
ginsenoside R2 + H2O
-
 Pyrococcus furiosus ginsenoside Rh1 + D-xylose
-
 ?
ginsenoside Re + H2O ginsenoside Re i.e. 20-(beta-D-glucopyranosyl)oxy-6alpha-[alpha-L-rhamnopyranosyl-(1->2)-beta-D-glucopyranosyloxy]dammar-24-en-3beta,12beta-diol Pyrococcus furiosus ginsenoside Rg1 + L-rhamnose ginsenoside Rg1 i.e. 6alpha,20-bis(beta-D-glucopyranosyl)oxy-dammar-24-en-3beta,12beta-diol ?
ginsenoside Rf + H2O
-
 Pyrococcus furiosus ginsenoside Rh1 + D-glucose
-
 ?
ginsenoside Rg2 + H2O
-
 Pyrococcus furiosus ginsenoside Rh1 + L-rhamnose
-
 ?
additional information the enzyme hydrolyzes the outer glycoside at the C-6 position in protopanaxatriol-type ginsenosides whereas the enzyme does not hydrolyze the inner glucoside at the C-6 position and the glucoside at the C-20 position Pyrococcus furiosus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
 assay at Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Pyrococcus furiosus