Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Thermotoga neapolitana |
Crystallization (Comment) | Organism |
---|---|
at a resolution of 2.05A. It is composed of an (alpha/beta)8 domain similar to a triose phosphate isomerase barrel, a five-stranded alpha/beta sandwich domain (both of which are important for active-site organization), and a C-terminal fibronectin type III | Thermotoga neapolitana |
Protein Variants | Comment | Organism |
---|---|---|
D242G | mutant loses 96% of wild-type activity, activity is partially restored after addition of NaN3 | Thermotoga neapolitana |
D58A | dramatic decrease in kcat compared to wild-type, activity is highly recovered after addition of NaN3 | Thermotoga neapolitana |
E458G | dramatic decrease in kcat compared to wild-type, activity is partially restored after addition of NaN3 | Thermotoga neapolitana |
W243F | Km (4-nitrophenyl beta-D-glucopyranoside) close to wild-type, kcat decreased to 34% compared to wild-type | Thermotoga neapolitana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant D58A, pH 5.6, 90°C, 0.1 M NaN3 | Thermotoga neapolitana | |
0.047 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant E458G, pH 5.6, 90°C, 0.1 M NaN3 | Thermotoga neapolitana | |
0.06 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant D242G, pH 5.6, 90°C, 0.1 M NaN3 | Thermotoga neapolitana | |
0.06 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant E458G, pH 5.6, 90°C | Thermotoga neapolitana | |
0.06 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, pH 5.6, 90°C | Thermotoga neapolitana | |
0.08 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant D58A, pH 5.6, 90°C | Thermotoga neapolitana | |
0.09 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant W243F, pH 5.6, 90°C | Thermotoga neapolitana | |
0.17 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, pH 5.6, 90°C, 0.1 M NaN3 | Thermotoga neapolitana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga neapolitana | Q0GC07 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermotoga neapolitana | 4-nitrophenol + D-glucopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-glucosidase | - |
Thermotoga neapolitana |
Bgl3B | - |
Thermotoga neapolitana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant E458G, pH 5.6, 90°C | Thermotoga neapolitana | |
7.1 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant D58A, pH 5.6, 90°C | Thermotoga neapolitana | |
7.4 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant D58A, pH 5.6, 90°C, 0.1 M NaN3 | Thermotoga neapolitana | |
13 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant E458G, pH 5.6, 90°C, 0.1 M NaN3 | Thermotoga neapolitana | |
25.3 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant D242G, pH 5.6, 90°C, 0.1 M NaN3 | Thermotoga neapolitana | |
78 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, pH 5.6, 90°C, 0.1 M NaN3 | Thermotoga neapolitana | |
89.6 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant W243F, pH 5.6, 90°C | Thermotoga neapolitana | |
129 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, pH 5.6, 90°C | Thermotoga neapolitana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
assay at, highest activity | Thermotoga neapolitana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
61.6 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant E458G, pH 5.6, 90°C | Thermotoga neapolitana | |
88 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant D58A, pH 5.6, 90°C | Thermotoga neapolitana | |
173 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant D58A, pH 5.6, 90°C, 0.1 M NaN3 | Thermotoga neapolitana | |
273 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant E458G, pH 5.6, 90°C, 0.1 M NaN3 | Thermotoga neapolitana | |
435 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant D242G, pH 5.6, 90°C, 0.1 M NaN3 | Thermotoga neapolitana | |
446 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, pH 5.6, 90°C, 0.1 M NaN3 | Thermotoga neapolitana | |
996 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant W243F, pH 5.6, 90°C | Thermotoga neapolitana | |
2002 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, pH 5.6, 90°C | Thermotoga neapolitana |