Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.21 extracted from

  • Pozzo, T.; Pasten, J.L.; Karlsson, E.N.; Logan, D.T.
    Structural and functional analyses of beta-glucosidase 3B from Thermotoga neapolitana: a thermostable three-domain representative of glycoside hydrolase 3 (2010), J. Mol. Biol., 397, 724-739.
    View publication on PubMed
Search for more literature for 3.2.1.21

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His-tagged fusion protein Thermotoga neapolitana

Crystallization (Commentary)

Crystallization (Comment) Organism
at a resolution of 2.05A. It is composed of an (alpha/beta)8 domain similar to a triose phosphate isomerase barrel, a five-stranded alpha/beta sandwich domain (both of which are important for active-site organization), and a C-terminal fibronectin type III Thermotoga neapolitana

Protein Variants

Protein Variants Comment Organism
D242G mutant loses 96% of wild-type activity, activity is partially restored after addition of NaN3 Thermotoga neapolitana
D58A dramatic decrease in kcat compared to wild-type, activity is highly recovered after addition of NaN3 Thermotoga neapolitana
E458G dramatic decrease in kcat compared to wild-type, activity is partially restored after addition of NaN3 Thermotoga neapolitana
W243F Km (4-nitrophenyl beta-D-glucopyranoside) close to wild-type, kcat decreased to 34% compared to wild-type Thermotoga neapolitana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
 4-nitrophenyl beta-D-glucopyranoside mutant D58A, pH 5.6, 90°C, 0.1 M NaN3 Thermotoga neapolitana
0.047
-
 4-nitrophenyl beta-D-glucopyranoside mutant E458G, pH 5.6, 90°C, 0.1 M NaN3 Thermotoga neapolitana
0.06
-
 4-nitrophenyl beta-D-glucopyranoside mutant D242G, pH 5.6, 90°C, 0.1 M NaN3 Thermotoga neapolitana
0.06
-
 4-nitrophenyl beta-D-glucopyranoside mutant E458G, pH 5.6, 90°C Thermotoga neapolitana
0.06
-
 4-nitrophenyl beta-D-glucopyranoside wild-type, pH 5.6, 90°C Thermotoga neapolitana
0.08
-
 4-nitrophenyl beta-D-glucopyranoside mutant D58A, pH 5.6, 90°C Thermotoga neapolitana
0.09
-
 4-nitrophenyl beta-D-glucopyranoside mutant W243F, pH 5.6, 90°C Thermotoga neapolitana
0.17
-
 4-nitrophenyl beta-D-glucopyranoside wild-type, pH 5.6, 90°C, 0.1 M NaN3 Thermotoga neapolitana

Organism

Organism UniProt Comment Textmining
Thermotoga neapolitana Q0GC07
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermotoga neapolitana 4-nitrophenol + D-glucopyranose
-
 ?

Synonyms

Synonyms Comment Organism
beta-glucosidase
-
 Thermotoga neapolitana
Bgl3B
-
 Thermotoga neapolitana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6
-
 4-nitrophenyl beta-D-glucopyranoside mutant E458G, pH 5.6, 90°C Thermotoga neapolitana
7.1
-
 4-nitrophenyl beta-D-glucopyranoside mutant D58A, pH 5.6, 90°C Thermotoga neapolitana
7.4
-
 4-nitrophenyl beta-D-glucopyranoside mutant D58A, pH 5.6, 90°C, 0.1 M NaN3 Thermotoga neapolitana
13
-
 4-nitrophenyl beta-D-glucopyranoside mutant E458G, pH 5.6, 90°C, 0.1 M NaN3 Thermotoga neapolitana
25.3
-
 4-nitrophenyl beta-D-glucopyranoside mutant D242G, pH 5.6, 90°C, 0.1 M NaN3 Thermotoga neapolitana
78
-
 4-nitrophenyl beta-D-glucopyranoside wild-type, pH 5.6, 90°C, 0.1 M NaN3 Thermotoga neapolitana
89.6
-
 4-nitrophenyl beta-D-glucopyranoside mutant W243F, pH 5.6, 90°C Thermotoga neapolitana
129
-
 4-nitrophenyl beta-D-glucopyranoside wild-type, pH 5.6, 90°C Thermotoga neapolitana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
 assay at, highest activity Thermotoga neapolitana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
61.6
-
 4-nitrophenyl beta-D-glucopyranoside mutant E458G, pH 5.6, 90°C Thermotoga neapolitana
88
-
 4-nitrophenyl beta-D-glucopyranoside mutant D58A, pH 5.6, 90°C Thermotoga neapolitana
173
-
 4-nitrophenyl beta-D-glucopyranoside mutant D58A, pH 5.6, 90°C, 0.1 M NaN3 Thermotoga neapolitana
273
-
 4-nitrophenyl beta-D-glucopyranoside mutant E458G, pH 5.6, 90°C, 0.1 M NaN3 Thermotoga neapolitana
435
-
 4-nitrophenyl beta-D-glucopyranoside mutant D242G, pH 5.6, 90°C, 0.1 M NaN3 Thermotoga neapolitana
446
-
 4-nitrophenyl beta-D-glucopyranoside wild-type, pH 5.6, 90°C, 0.1 M NaN3 Thermotoga neapolitana
996
-
 4-nitrophenyl beta-D-glucopyranoside mutant W243F, pH 5.6, 90°C Thermotoga neapolitana
2002
-
 4-nitrophenyl beta-D-glucopyranoside wild-type, pH 5.6, 90°C Thermotoga neapolitana