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Literature summary for 3.2.1.21 extracted from
- Beta-glycosidase of Thermus thermophilus KNOUC202: gene and biochemical properties of the enzyme expressed in Escherichia coli (2010), Appl. Biochem. Microbiol., 46, 515-524.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Thermus thermophilus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.4, 75°C | Thermus thermophilus |  |
| 4.84 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 5.4, 75°C | Thermus thermophilus | |
| 6.05 | - |
2-nitrophenyl-beta-D-galactopyranoside | pH 5.4, 75°C | Thermus thermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48660 | - |
deduced from cDNA | Thermus thermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | Q0GA07 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using gel filtration and ion-exchange chromatography | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Thermus thermophilus | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
| 4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Thermus thermophilus | 4-nitrophenol + beta-D-fucopyranose | - |
? | |
| 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Thermus thermophilus | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermus thermophilus | 4-nitrophenol + D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-lactopyranoside + H2O | - |
Thermus thermophilus | 4-nitrophenol + D-lactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | gel filtration | Thermus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-glucosidase | - |
Thermus thermophilus |
| KNOUC202beta-gly | - |
Thermus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
assay at | Thermus thermophilus |
| 90 | - |
optimal temperature | Thermus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
enzyme shows high thermostability not losing its activity at 80°C for 2 h in 0.05 M Na-phosphate buffer of pH 6.8 with Tm of 100.0°C in 0.02 M Tris-HCl buffer of pH 8.2 | Thermus thermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 32.3 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.4, 75°C | Thermus thermophilus | |
| 167.4 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 5.4, 75°C | Thermus thermophilus | |
| 288.3 | - |
2-nitrophenyl-beta-D-galactopyranoside | pH 5.4, 75°C | Thermus thermophilus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
optimal pH | Thermus thermophilus |
| 7 | - |
assay at | Thermus thermophilus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 34.6 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 5.4, 75°C | Thermus thermophilus | |
| 47.7 | - |
2-nitrophenyl-beta-D-galactopyranoside | pH 5.4, 75°C | Thermus thermophilus | |
| 700.6 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 5.4, 75°C | Thermus thermophilus | |
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