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Literature summary for 3.2.1.21 extracted from

  • Wakuta, S.; Hamada, S.; Ito, H.; Matsuura, H.; Nabeta, K.; Matsui, H.
    Identification of a beta-glucosidase hydrolyzing tuberonic acid glucoside in rice (Oryza sativa L.) (2010), Phytochemistry, 71, 1280-1288.
    View publication on PubMed
Search for more literature for 3.2.1.21

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 tuberonic acid beta-D-glucoside pH 4.0, 37°C Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 1 * 42000 + 1 * 26000, SDS-PAGE Oryza sativa
42000
-
 1 * 42000 + 1 * 26000, SDS-PAGE Oryza sativa
65000
-
 gel filtration Oryza sativa
66000
-
 native-PAGE Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Oryza sativa

Purification (Commentary)

Purification (Comment) Organism
ca. 4300fold, yield of 1.1%, ammonium sulfate precipitation, CM-Sepharose, ConA Sepharose, Superdex 200, Resource S, hydroxyapatite Oryza sativa

Source Tissue

Source Tissue Comment Organism Textmining
inflorescence during grain-filling Oryza sativa
-
leaf
-
 Oryza sativa
-
seed purification of OsTAGG1 from immature rice seeds Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.73
-
 tuberonic acid beta-D-glucoside as substrate, 4300fold purified protein, pH 4.0, 37°C Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-O-beta-D-glucopyranosylsalicylate + H2O 25% relative activity compared to hydrolysis of tuberonic acid beta-D-glucoside Oryza sativa salicylic acid + beta-D-glucose
-
 ?
4-nitrophenyl beta-D-glucoside + H2O 178% relative activity compared to hydrolysis of tuberonic acid beta-D-glucoside Oryza sativa 4-nitrophenol + beta-D-glucose
-
 ?
methyl tuberonic acid beta-D-glucoside + H2O i.e. 12-hydroxyjasmonic acid beta-d-glucosid methyl ester, 80% relative activity compared to hydrolysis of tuberonic acid beta-D-glucoside Oryza sativa methyl tuberonic acid + beta-D-glucose
-
 ?
additional information jasmonic acid beta-D-glucosyl ester + H2O, product: jasmonic acid + beta-D-glucose, 51% relative activity compared to hydrolysis of tuberonic acid beta-D-glucoside Oryza sativa ?
-
 ?
additional information no or negligible hydrolyzing activity with zeatin-O-glucoside, linamarin, or 4-nitrophenyl-beta-D-mannoside, 4-nitrophenyl-beta-D-galactoside, 4-nitrophenyl-beta-D-cellobioside, and 4-nitrophenyl-alpha-D-glucoside Oryza sativa ?
-
 ?
tuberonic acid beta-D-glucoside + H2O
-
 Oryza sativa tuberonic acid + beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 42000 + 1 * 26000, SDS-PAGE Oryza sativa

Synonyms

Synonyms Comment Organism
OsTAGG OsTAGG1 and OsTAGG2, detected by CM-Sepharose chromatography, at least two isoforms in rice panicles Oryza sativa
tuberonic acid glucoside (TAG)-hydrolyzing beta-glucosidase a specific beta-glucosidase hydrolyzing tuberonic acid beta-D-glucose Oryza sativa
tuberonic acid glucoside beta-glucosidase
-
 Oryza sativa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Oryza sativa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 after 15 min at various temperatures ranging from 30 to 70°C, the original activity retained up to 50°C Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Oryza sativa

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.3 10.3 fairly stable, 90% of the original activity retains after incubation at 4°C for 16 h Oryza sativa