Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | in the presence of 16% (v/v) ethanol the enzyme activity is increased more than 2fold | Levilactobacillus brevis | |
methanol | in the presence of 16% (v/v) methanol the enzyme activity is increased more than 2fold | Levilactobacillus brevis | |
additional information | high concentrations (up to 0.2 M) of fructose, malate, lactate, mannitol and sorbitol have little or no effect on the enzyme activity | Levilactobacillus brevis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-gluconate | 17% of initial activity at 0.2 M | Levilactobacillus brevis | |
D-gluconic acid delta-lactone | 14% of initial activity at 10 mM | Levilactobacillus brevis | |
D-glucose | 59% of initial activity at 0.2 M | Levilactobacillus brevis | |
additional information | the enzyme is not inhibited by D-fructose | Levilactobacillus brevis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
4-nitrophenyl-beta-D-glucopyranoside | in 0.2 mM citrate-phosphate buffer, pH 5.5, at 37°C | Levilactobacillus brevis | |
1.14 | - |
4-nitrophenyl-beta-D-xylopyranoside | in 0.2 mM citrate-phosphate buffer, pH 5.5, at 37°C | Levilactobacillus brevis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Levilactobacillus brevis | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
4 * 80000, SDS-PAGE | Levilactobacillus brevis |
330000 | - |
gel filtration | Levilactobacillus brevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Levilactobacillus brevis | the pure glucosidase from Lactobacillus brevis has also side activities of xylosidase, arabinosidase, and cellobiosidase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Levilactobacillus brevis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Macro-Prep methyl HIC column chromatography, Source 15 Q column chromatography, and Sephacryl S-300 gel filtration | Levilactobacillus brevis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | - |
after 244fold purification, using 4-nitrophenyl-beta-D-glalactopyranoside substrate, as at 37°C | Levilactobacillus brevis |
0.29 | - |
crude extract, using 4-nitrophenyl-beta-D-glucopyranoside substrate, at 37°C | Levilactobacillus brevis |
3.5 | - |
after 244fold purification, using 4-nitrophenyl-alpha-L-arabinopyranoside substrate, as at 37°C | Levilactobacillus brevis |
9.4 | - |
after 244fold purification, using 4-nitrophenyl-beta-D-cellobioside substrate, as at 37°C | Levilactobacillus brevis |
33 | - |
after 244fold purification, using 4-nitrophenyl-beta-D-xylopyranoside substrate, as at 37°C | Levilactobacillus brevis |
70.9 | - |
after 244fold purification, using 4-nitrophenyl -beta-D-glucopyranoside substrate, as at 37°C | Levilactobacillus brevis |
Storage Stability | Organism |
---|---|
4°C, in 20 mM citrate-phosphate buffer, half-lives of 4 days at pH 4.0 and 50 days at pH 7.0 | Levilactobacillus brevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-cellobioside + H2O | 13% activity compared to 4-nitrophenyl beta-D-glucopyranoside | Levilactobacillus brevis | 4-nitrophenol + cellobiose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | 0.1% activity compared to 4-nitrophenyl beta-D-glucopyranoside | Levilactobacillus brevis | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | 100% activity | Levilactobacillus brevis | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | 46% activity compared to 4-nitrophenyl beta-D-glucopyranoside | Levilactobacillus brevis | 4-nitrophenol + beta-D-xylose | - |
? | |
4-nitrophenyl-alpha-L-arabinopyranoside + H2O | 4.9% activity compared to 4-nitrophenyl beta-D-glucopyranoside | Levilactobacillus brevis | 4-nitrophenol + alpha-L-arabinopyranose | - |
? | |
cellobiose + H2O | - |
Levilactobacillus brevis | 2 beta-D-glucose | - |
? | |
additional information | the pure glucosidase from Lactobacillus brevis has also side activities of xylosidase, arabinosidase, and cellobiosidase | Levilactobacillus brevis | ? | - |
? | |
additional information | no hydrolysis of 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-alpha-L-rhamnopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-alpha-D-xylopyranoside, 4-nitrophenyl-alpha-D-mannopyranoside, and 4-nitrophenyl-alpha-D-galactopyranoside | Levilactobacillus brevis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 80000, SDS-PAGE | Levilactobacillus brevis |
Synonyms | Comment | Organism |
---|---|---|
beta-D-glucosidase | - |
Levilactobacillus brevis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Levilactobacillus brevis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | the enzyme displays good stability when it is kept at 25 and 37°C, with approximate half-lives of 160 and 3.5 h, respectively. When the enzyme is kept at 45°C, less than 5% of initial activity is detectable after 30 min | Levilactobacillus brevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Levilactobacillus brevis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | the free enzyme is stable at pH 7.0 (half-life of 50 days) but not at pH 4.0 (half-life of 4 days) | Levilactobacillus brevis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Levilactobacillus brevis | isoelectric focusing | - |
3.5 |