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Literature summary for 3.2.1.21 extracted from

  • Ghorai, S.; Chowdhury, S.; Pal, S.; Banik, S.P.; Mukherjee, S.; Khowala, S.
    Enhanced activity and stability of cellobiase (beta-glucosidase: EC 3.2.1.21) produced in the presence of 2-deoxy-D-glucose from the fungus Termitomyces clypeatus (2010), Carbohydr. Res., 345, 1015-1022.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 129.9% relative activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 1-2 mM Termitomyces clypeatus

General Stability

General Stability Organism
less glycosylated cellobiase is resistant towards proteolytic as well as endoglycosidase-H digestion and shows higher stability than native enzyme due to increased aggregation of the protein Termitomyces clypeatus

Inhibitors

Inhibitors Comment Organism Structure
2-deoxy-D-glucose 82.7% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 1 mg/ml Termitomyces clypeatus
4-chloromercuribenzoate 63.1% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 0.5 mM Termitomyces clypeatus
beta-mercaptoethanol 79-22% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 10-20 mM Termitomyces clypeatus
Cu2+ 78.4% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 2.0 mM Termitomyces clypeatus
D-glucose 14.6% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 20 mg/ml Termitomyces clypeatus
EDTA 69.1% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 20 mM Termitomyces clypeatus
guanidine hydrochloride 83.3-45.5% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 1-2 M Termitomyces clypeatus
Hg2+ 40.9% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 2.0 mM Termitomyces clypeatus
KCl 80.3% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 20 mM Termitomyces clypeatus
Mn2+ 54.7% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 20 mM Termitomyces clypeatus
Sodium azide 77.8% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 20 mM Termitomyces clypeatus
Urea 69% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 4 M Termitomyces clypeatus
Zn2+ 67.5% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 2.0 mM Termitomyces clypeatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.187
-
4-nitrophenyl-beta-D-glucopyranoside purified enzyme, in 0.1 M NaOAc buffer, pH 5.0, at 45°C Termitomyces clypeatus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Termitomyces clypeatus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57300
-
high-performance gel-permeation liquid chromatography Termitomyces clypeatus
116000
-
SDS-PAGE Termitomyces clypeatus

Organism

Organism UniProt Comment Textmining
Termitomyces clypeatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified in the presence and absence of 2-deoxy-D-glucose by ultrafiltration, DEAE Sephadex A-50 gel filtration, high-performance gel-permeation liquid chromatography, and Sephacryl S-200 gel filtration Termitomyces clypeatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.9
-
purified enzyme, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 0.1 M NaOAc buffer, pH 5.0, at 45°C Termitomyces clypeatus
57.14
-
crude filtrate, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 0.1 M NaOAc buffer, pH 5.0, at 45°C Termitomyces clypeatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Termitomyces clypeatus 4-nitrophenol + D-glucopyranose
-
?
cellobiose + H2O
-
Termitomyces clypeatus 2 beta-D-glucose
-
?
additional information does not hydrolyze 2-nitrophenyl alpha-D-galactopyranoside, methyl beta-D-glucopyranoside, carboxymethylcellulose, methyl alpha-D-galactopyranoside, maltose, and trehalose Termitomyces clypeatus ?
-
?

Synonyms

Synonyms Comment Organism
cellobiase
-
Termitomyces clypeatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Termitomyces clypeatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60
-
Termitomyces clypeatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
-
Termitomyces clypeatus

pH Range

pH Minimum pH Maximum Comment Organism
3 8
-
Termitomyces clypeatus