Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 129.9% relative activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 1-2 mM | Termitomyces clypeatus |
General Stability | Organism |
---|---|
less glycosylated cellobiase is resistant towards proteolytic as well as endoglycosidase-H digestion and shows higher stability than native enzyme due to increased aggregation of the protein | Termitomyces clypeatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-deoxy-D-glucose | 82.7% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 1 mg/ml | Termitomyces clypeatus | |
4-chloromercuribenzoate | 63.1% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 0.5 mM | Termitomyces clypeatus | |
beta-mercaptoethanol | 79-22% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 10-20 mM | Termitomyces clypeatus | |
Cu2+ | 78.4% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 2.0 mM | Termitomyces clypeatus | |
D-glucose | 14.6% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 20 mg/ml | Termitomyces clypeatus | |
EDTA | 69.1% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 20 mM | Termitomyces clypeatus | |
guanidine hydrochloride | 83.3-45.5% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 1-2 M | Termitomyces clypeatus | |
Hg2+ | 40.9% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 2.0 mM | Termitomyces clypeatus | |
KCl | 80.3% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 20 mM | Termitomyces clypeatus | |
Mn2+ | 54.7% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 20 mM | Termitomyces clypeatus | |
Sodium azide | 77.8% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 20 mM | Termitomyces clypeatus | |
Urea | 69% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 4 M | Termitomyces clypeatus | |
Zn2+ | 67.5% residual activity of cellobiase produced in the presence of 2-deoxy-D-glucose at 2.0 mM | Termitomyces clypeatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.187 | - |
4-nitrophenyl-beta-D-glucopyranoside | purified enzyme, in 0.1 M NaOAc buffer, pH 5.0, at 45°C | Termitomyces clypeatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Termitomyces clypeatus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57300 | - |
high-performance gel-permeation liquid chromatography | Termitomyces clypeatus |
116000 | - |
SDS-PAGE | Termitomyces clypeatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Termitomyces clypeatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purified in the presence and absence of 2-deoxy-D-glucose by ultrafiltration, DEAE Sephadex A-50 gel filtration, high-performance gel-permeation liquid chromatography, and Sephacryl S-200 gel filtration | Termitomyces clypeatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
17.9 | - |
purified enzyme, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 0.1 M NaOAc buffer, pH 5.0, at 45°C | Termitomyces clypeatus |
57.14 | - |
crude filtrate, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 0.1 M NaOAc buffer, pH 5.0, at 45°C | Termitomyces clypeatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Termitomyces clypeatus | 4-nitrophenol + D-glucopyranose | - |
? | |
cellobiose + H2O | - |
Termitomyces clypeatus | 2 beta-D-glucose | - |
? | |
additional information | does not hydrolyze 2-nitrophenyl alpha-D-galactopyranoside, methyl beta-D-glucopyranoside, carboxymethylcellulose, methyl alpha-D-galactopyranoside, maltose, and trehalose | Termitomyces clypeatus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cellobiase | - |
Termitomyces clypeatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Termitomyces clypeatus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | - |
Termitomyces clypeatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
- |
Termitomyces clypeatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | - |
Termitomyces clypeatus |