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Literature summary for 3.2.1.21 extracted from

  • Kim, H.J.; Park, A.R.; Lee, J.K.; Oh, D.K.
    Characterization of an acid-labile, thermostable beta-glycosidase from Thermoplasma acidophilum (2009), Biotechnol. Lett., 31, 1457-1462.
    View publication on PubMed
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by EDTA Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 4-nitrophenyl-beta-D-fucopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum
0.4
-
 4-nitrophenyl-beta-D-glucopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum
1.3
-
 4-nitrophenyl-beta-D-mannopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum
1.5
-
 4-nitrophenyl-beta-D-galactopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is not activated by the presence of monovalent or divalent cations Thermoplasma acidophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 2 * 57000, SDS-PAGE Thermoplasma acidophilum
57070
-
 subunit, MALDI-TOF mass spectrometry Thermoplasma acidophilum
114000
-
 native enzyme, gel filtration Thermoplasma acidophilum

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum Q9HIL6 strain DSM 1728
-

Purification (Commentary)

Purification (Comment) Organism
Hi-Trap column chromatography, gel filtration Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.7
-
 after 68fold purification, using 2-nitrophenyl-beta-D-galactopyranoside as substrate, at pH 6.0 and 90°C Thermoplasma acidophilum
1.2
-
 crude extract, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 6.0 and 90°C Thermoplasma acidophilum
1.7
-
 after 68fold purification, using 2-nitrophenyl-beta-D-fucopyranoside as substrate, at pH 6.0 and 90°C Thermoplasma acidophilum
1.8
-
 after 68fold purification, using 2-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 6.0 and 90°C Thermoplasma acidophilum
4.9
-
 after 68fold purification, using 4-nitrophenyl-beta-D-mannopyranoside as substrate, at pH 6.0 and 90°C Thermoplasma acidophilum
8.5
-
 after 68fold purification, using 4-nitrophenyl-beta-D-galactopyranoside as substrate, at pH 6.0 and 90°C Thermoplasma acidophilum
82
-
 after 68fold purification, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at pH 6.0 and 90°C Thermoplasma acidophilum
113
-
 after 68fold purification, using 4-nitrophenyl-beta-D-fucopyranoside as substrate, at pH 6.0 and 90°C Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-fucopyranoside + H2O
-
 Thermoplasma acidophilum 4-nitrophenol + beta-D-fucopyranose
-
 ?
4-nitrophenyl beta-D-galactoside + H2O
-
 Thermoplasma acidophilum 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermoplasma acidophilum 4-nitrophenol + D-glucopyranose
-
 ?
4-nitrophenyl beta-D-mannopyranoside + H2O
-
 Thermoplasma acidophilum 4-nitrophenol + beta-D-mannopyranose
-
 ?
additional information no activity is detected for 4-nitrophenyl-beta-D-xylopyranoside, 4-nitrophenyl-beta-L-arabinopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, and 4-nitrophenyl-alpha-L-arabinopyranoside Thermoplasma acidophilum ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 57000, SDS-PAGE Thermoplasma acidophilum

Synonyms

Synonyms Comment Organism
BglA
-
 Thermoplasma acidophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Thermoplasma acidophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14
-
 4-nitrophenyl-beta-D-mannopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum
27
-
 4-nitrophenyl-beta-D-galactopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum
141
-
 4-nitrophenyl-beta-D-glucopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum
182
-
 4-nitrophenyl-beta-D-fucopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 the maximum activity of BglA at 75°C is initially at pH 4.5 but after 1 h at 75°C is at pH 6.0 Thermoplasma acidophilum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 6 the enzyme is unstable below pH 6.0, at pH 6.0 its half-life at 75°C is 28 days but at pH 4.5 is 13 h Thermoplasma acidophilum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
11
-
 4-nitrophenyl-beta-D-mannopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum
18
-
 4-nitrophenyl-beta-D-galactopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum
381
-
 4-nitrophenyl-beta-D-glucopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum
1068
-
 4-nitrophenyl-beta-D-fucopyranoside in 100 mM citrate/phosphate buffer, at pH 6.0 and 90°C Thermoplasma acidophilum