Cloned (Comment) | Organism |
---|---|
expressed in Aspergillus oryzae | Penicillium brasilianum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-nitrophenyl-beta-D-glucopyranoside | substrate inhibition | Penicillium brasilianum | |
D-glucono-delta-lactone | - |
Penicillium brasilianum | |
D-glucose | - |
Penicillium brasilianum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
4-nitrophenyl-beta-D-glucopyranoside | recombinant enzyme, in 50 mM sodium citrate buffer pH 4.8, at 22°C | Penicillium brasilianum | |
1.58 | - |
cellobiose | recombinant enzyme, in 50 mM sodium citrate buffer pH 4.8, at 22°C | Penicillium brasilianum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
115000 | - |
SDS-PAGE | Penicillium brasilianum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium brasilianum | A5A4M8 | - |
- |
Penicillium brasilianum IBT 20888 | A5A4M8 | - |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose column chromatography, Superdex 75 gel filtration, Resource Q column chromatography, Source 30S column chromatography, Superdex 200 gel filtration, and phenyl Sepharose column chromatography | Penicillium brasilianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Penicillium brasilianum | 4-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Penicillium brasilianum IBT 20888 | 4-nitrophenol + D-glucopyranose | - |
? | |
cellobiose + H2O | BGL has significant lower affinity for cellobiose compared with 4-nitrophenyl-beta-D-glucopyranoside | Penicillium brasilianum | 2 beta-D-glucose | - |
? | |
cellobiose + H2O | BGL has significant lower affinity for cellobiose compared with 4-nitrophenyl-beta-D-glucopyranoside | Penicillium brasilianum IBT 20888 | 2 beta-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Bgl | - |
Penicillium brasilianum |
GH3 beta-glucosidase | - |
Penicillium brasilianum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
at pH values 4.16, 4.80 and 6.17 | Penicillium brasilianum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
no loss in activity after 24 h of incubation at 60°C at pH 4-6 | Penicillium brasilianum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
43 | - |
cellobiose | recombinant enzyme, in 50 mM sodium citrate buffer pH 4.8, at 22°C | Penicillium brasilianum | |
118 | - |
4-nitrophenyl-beta-D-glucopyranoside | recombinant enzyme, in 50 mM sodium citrate buffer pH 4.8, at 22°C | Penicillium brasilianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.8 | - |
- |
Penicillium brasilianum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | stable in the range of pH 4.0 to 6.0 while outside this pH range no residual activity is measured, at 65°C the residual activity is 50% at pH 5.0 and 6.0 (after 24 h) and 20% at pH 4.0 | Penicillium brasilianum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
D-glucono-delta-lactone | recombinant enzyme, using cellobiose as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C | Penicillium brasilianum | |
0.1 | - |
D-glucono-delta-lactone | recombinant enzyme, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C | Penicillium brasilianum | |
1.1 | - |
D-glucose | recombinant enzyme, using cellobiose as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C | Penicillium brasilianum | |
2.3 | - |
D-glucose | recombinant enzyme, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, in 50 mM sodium citrate buffer pH 4.8, at 22°C | Penicillium brasilianum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Penicillium brasilianum | isoelectric focusing | - |
3.9 |