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Literature summary for 3.2.1.21 extracted from

  • Daroit, D.J.; Simonetti, A.; Hertz, P.F.; Brandelli, A.
    Purification and characterization of an extracellular beta-glucosidase from Monascus purpureus (2008), J. Microbiol. Biotechnol., 18, 933-941.
    View publication on PubMed
Search for more literature for 3.2.1.21

Activating Compound

Activating Compound Comment Organism Structure
ethanol slight stimulation at low concentrations Monascus purpureus
methanol slight stimulation at low concentrations Monascus purpureus

Inhibitors

Inhibitors Comment Organism Structure
D-glucose 10 mM, 42% residual activity Monascus purpureus
HgCl2 5 mM, 14% residual activity Monascus purpureus
K2Cr2O7 5 mM, 49% residual activity Monascus purpureus
K2CrO4 5 mM, 80% residual activity Monascus purpureus
additional information not inhibitory: 2-mercaptoethanol, sodium dodecyl sulfate, EDTA Monascus purpureus

Organism

Organism UniProt Comment Textmining
Monascus purpureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Monascus purpureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
84.3
-
 pH 4.5, 37°C Monascus purpureus

Storage Stability

Storage Stability Organism
-15°C, 60 days, no significant loss in activity Monascus purpureus
5°C, 60 days, no significant loss in activity Monascus purpureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + 2 H2O
-
 Monascus purpureus 4-nitrophenol + 2 D-glucose glucosyl residues are removed from the non-reducing end of substrate ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Monascus purpureus 4-nitrophenol + D-glucose
-
 ?
cellobiose + H2O
-
 Monascus purpureus 2 D-glucose
-
 ?
maltose + H2O at 125% of the rate with cellobiose Monascus purpureus 2 D-glucose
-
 ?
n-octyl-beta-D-glucopyranoside + H2O at 75% of the rate with cellobiose Monascus purpureus n-octanol + D-glucose
-
 ?
salicin + H2O at 70% of the rate with cellobiose Monascus purpureus salicyl alcohol + D-glucose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Monascus purpureus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 2 h, completely stable Monascus purpureus
60
-
 1 h, 83% residual activity Monascus purpureus
70
-
 half-life 1.8 min Monascus purpureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Monascus purpureus