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Literature summary for 3.2.1.21 extracted from

  • Nomura, T.; Quesada, A.L.; Kutchan, T.M.
    The new beta-D-glucosidase in terpenoid-isoquinoline alkaloid biosynthesis in Psychotria ipecacuanha (2008), J. Biol. Chem., 283, 34650-34659.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.21

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 50% residual activity Carapichea ipecacuanha
Fe3+ 1 mM, 46% residual activity Carapichea ipecacuanha
additional information not inhibitory: EDTA, 2-mercaptoethanol Carapichea ipecacuanha
Zn2+ 1 mM, 64% residual activity Carapichea ipecacuanha

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 ipecoside pH 5.0, 30°C Carapichea ipecacuanha
0.19
-
 strictosidine pH 5.0, 30°C Carapichea ipecacuanha
0.29
-
 vincoside pH 5.0, 30°C Carapichea ipecacuanha
0.32
-
 N-deacetylipecoside pH 5.0, 30°C Carapichea ipecacuanha
0.39
-
 6-O-methyl-N-deacetylipecoside pH 5.0, 30°C Carapichea ipecacuanha

Organism

Organism UniProt Comment Textmining
Carapichea ipecacuanha B6ZKM3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-O-methyl-N-deacetylipecoside + H2O 90.5% of the efficiency with N-deacetylipecoside Carapichea ipecacuanha ?
-
 ?
ipecoside + H2O 586% of the efficiency with N-deacetylipecoside Carapichea ipecacuanha ?
-
 ?
additional information isoform IpeGlu1 preferentially hydrolyzes glucosidic Ipecac alkaloids except for their lactams, but shows poor or no activity toward other substrates, including terpenoid-indole alkaloid glucosides. IpeGlu1 activity is extremely poor toward 7-O-methyl and 6,7-O,O-dimethyl derivatives. 6-O-methyl derivatives are hydrolyzed as efficiently as non-methylated substrates. IpeGlu1 lacks stereospecificity for its substrates. 1(R)-epimers are preferred to 1(S)-epimers Carapichea ipecacuanha ?
-
 ?
N-deacetylipecoside + H2O
-
 Carapichea ipecacuanha ?
-
 ?
strictosidine + H2O 12.6% of the efficiency with N-deacetylipecoside Carapichea ipecacuanha ?
-
 ?
vincoside + H2O 25.1% of the efficiency with N-deacetylipecoside Carapichea ipecacuanha ?
-
 ?

Synonyms

Synonyms Comment Organism
Glu1
-
 Carapichea ipecacuanha

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
 strictosidine pH 5.0, 30°C Carapichea ipecacuanha
6.4
-
 vincoside pH 5.0, 30°C Carapichea ipecacuanha
28.2
-
 N-deacetylipecoside pH 5.0, 30°C Carapichea ipecacuanha
31.1
-
 6-O-methyl-N-deacetylipecoside pH 5.0, 30°C Carapichea ipecacuanha
51.6
-
 ipecoside pH 5.0, 30°C Carapichea ipecacuanha