Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | up to 10% v/v, increase in activity to 120% | Metschnikowia pulcherrima | |
Fe3+ | 10 mM, 115% of initial activity | Metschnikowia pulcherrima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | enzyme is active and stable in presence of 5 g glucose per l. In presence of 100 g glucose per l, 25% residual activity | Metschnikowia pulcherrima | |
Mg2+ | 10 mM, 22% inhibition | Metschnikowia pulcherrima | |
additional information | not inhibitory: EDTA | Metschnikowia pulcherrima | |
Zn2+ | 10 mM, complete inhibition | Metschnikowia pulcherrima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 4.5, 23°C | Metschnikowia pulcherrima |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
x * 49000, SDS-PAGE | Metschnikowia pulcherrima |
400000 | - |
gel filtration | Metschnikowia pulcherrima |
Organic Solvent | Comment | Organism |
---|---|---|
Ethanol | enzyme is active and stable in presence of 12% v/v ethanol and 5 g per l glucose. Up to 10% ethanol, increase in activity to 120% | Metschnikowia pulcherrima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metschnikowia pulcherrima | - |
best source of beta-glucosidase activity among 60 yeasts isolated from grape musts | - |
Purification (Comment) | Organism |
---|---|
- |
Metschnikowia pulcherrima |
Storage Stability | Organism |
---|---|
23°C, pH 4.5-7.0, half-life 5 h | Metschnikowia pulcherrima |
4°C, pH 4.5-7.0, half-life 50 h | Metschnikowia pulcherrima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Metschnikowia pulcherrima | 2-nitrophenol + beta-D-galactose | 2% of the activity with 4-nitrophenyl beta-D-glucopyranoside | ? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Metschnikowia pulcherrima | 4-nitrophenol + beta-D-glucose | - |
? | |
cellobiose + H2O | - |
Metschnikowia pulcherrima | 2 beta-D-glucose | 22% of the activity with 4-nitrophenyl beta-D-glucopyranoside | ? | |
additional information | enzyme is active against (1-4)beta and (1-2)beta glycosidic linkages | Metschnikowia pulcherrima | ? | - |
? | |
saccharose + H2O | - |
Metschnikowia pulcherrima | D-glucose + D-fructose | 5% of the activity with 4-nitrophenyl beta-D-glucopyranoside | ? |
Subunits | Comment | Organism |
---|---|---|
oligomer | x * 49000, SDS-PAGE | Metschnikowia pulcherrima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Metschnikowia pulcherrima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
pH 4.5-7.0, half-life 50 h | Metschnikowia pulcherrima |
23 | - |
pH 4.5-7.0, half-life 5 h | Metschnikowia pulcherrima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Metschnikowia pulcherrima |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | - |
unstable below | Metschnikowia pulcherrima |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Metschnikowia pulcherrima | isoelectric focusing | - |
4.2 |