Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.21 extracted from

  • Gonzalez-Pombo, P.; Perez, G.; Carrau, F.; Guisan, J.M.; Batista-Viera, F.; Brena, B.M.
    One-step purification and characterization of an intracellular beta-glucosidase from Metschnikowia pulcherrima (2008), Biotechnol. Lett., 30, 1469-1475.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ethanol up to 10% v/v, increase in activity to 120% Metschnikowia pulcherrima
Fe3+ 10 mM, 115% of initial activity Metschnikowia pulcherrima

Inhibitors

Inhibitors Comment Organism Structure
D-glucose enzyme is active and stable in presence of 5 g glucose per l. In presence of 100 g glucose per l, 25% residual activity Metschnikowia pulcherrima
Mg2+ 10 mM, 22% inhibition Metschnikowia pulcherrima
additional information not inhibitory: EDTA Metschnikowia pulcherrima
Zn2+ 10 mM, complete inhibition Metschnikowia pulcherrima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5
-
4-nitrophenyl beta-D-glucopyranoside pH 4.5, 23°C Metschnikowia pulcherrima

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
x * 49000, SDS-PAGE Metschnikowia pulcherrima
400000
-
gel filtration Metschnikowia pulcherrima

Organic Solvent Stability

Organic Solvent Comment Organism
Ethanol enzyme is active and stable in presence of 12% v/v ethanol and 5 g per l glucose. Up to 10% ethanol, increase in activity to 120% Metschnikowia pulcherrima

Organism

Organism UniProt Comment Textmining
Metschnikowia pulcherrima
-
best source of beta-glucosidase activity among 60 yeasts isolated from grape musts
-

Purification (Commentary)

Purification (Comment) Organism
-
Metschnikowia pulcherrima

Storage Stability

Storage Stability Organism
23°C, pH 4.5-7.0, half-life 5 h Metschnikowia pulcherrima
4°C, pH 4.5-7.0, half-life 50 h Metschnikowia pulcherrima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
Metschnikowia pulcherrima 2-nitrophenol + beta-D-galactose 2% of the activity with 4-nitrophenyl beta-D-glucopyranoside ?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Metschnikowia pulcherrima 4-nitrophenol + beta-D-glucose
-
?
cellobiose + H2O
-
Metschnikowia pulcherrima 2 beta-D-glucose 22% of the activity with 4-nitrophenyl beta-D-glucopyranoside ?
additional information enzyme is active against (1-4)beta and (1-2)beta glycosidic linkages Metschnikowia pulcherrima ?
-
?
saccharose + H2O
-
Metschnikowia pulcherrima D-glucose + D-fructose 5% of the activity with 4-nitrophenyl beta-D-glucopyranoside ?

Subunits

Subunits Comment Organism
oligomer x * 49000, SDS-PAGE Metschnikowia pulcherrima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Metschnikowia pulcherrima

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
pH 4.5-7.0, half-life 50 h Metschnikowia pulcherrima
23
-
pH 4.5-7.0, half-life 5 h Metschnikowia pulcherrima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
Metschnikowia pulcherrima

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
unstable below Metschnikowia pulcherrima

pI Value

Organism Comment pI Value Maximum pI Value
Metschnikowia pulcherrima isoelectric focusing
-
4.2