Cloned (Comment) | Organism |
---|---|
native and mutated cDNA sequences cloned into vector pPICZalphaB and expressed in Pichia pastoris | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
gluconolactone | competitively inhibits with p-nitrophenyl-beta-D-glucopyranoside, o-nitrophenyl-beta-D-glucopyranoside, and 4-methylumbelliferyl-beta-D-glucopyranoside as substrates | Arabidopsis thaliana | |
additional information | the enzyme is very tolerant to glucose inhibition | Arabidopsis thaliana | |
p-nitrophenyl-1-thio-beta-D-glucopyranoside | competitively inhibits with p-nitrophenyl-beta-D-glucopyranoside, o-nitrophenyl-beta-D-glucopyranoside, and 4-methylumbelliferyl-beta-D-glucopyranoside as substrates | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.78 | - |
4-methylumbelliferyl-beta-D-glucopyranoside | - |
Arabidopsis thaliana | |
1.9 | - |
p-nitrophenyl-beta-D-glucopyranoside | - |
Arabidopsis thaliana | |
2.1 | - |
o-nitrophenyl-beta-D-glucopyranoside | - |
Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE, recombinant mutated beta-glucosidase | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SLA0 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant beta-glucosidase purified by gel filtration to apparent homogeneity, 21fold with an overall enzyme yield of 6.8% | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.34 | - |
21fold purified recombinant beta-glucosidase | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-glucopyranoside + H2O | 95% relative activity | Arabidopsis thaliana | 2-nitrophenol + D-glucopyranose | - |
? | |
4-methylumbelliferyl-beta-D-glucopyranoside + H2O | 163% relative activity | Arabidopsis thaliana | 4-methylumbelliferol + beta-D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | 100% relative activity | Arabidopsis thaliana | 4-nitrophenol + D-glucopyranose | - |
? | |
amygdalin + H2O | 48% relative activity | Arabidopsis thaliana | prunasin + D-glucose | - |
? | |
beta-gentiobiose + H2O | 6.3% relative activity | Arabidopsis thaliana | ? | - |
? | |
D(+)cellobiose + H2O | 6.3% relative activity | Arabidopsis thaliana | ? | - |
? | |
additional information | necessity of the nucleophilic glutamic acid residue, located in the motif YITENG, for the substrate hydrolysis. The mutated recombinant beta-glucosidase isoenzyme shows no activity with p-nitrophenyl 1-thio-beta-D-glucopyranoside, p-nitrophenyl beta-D-mannopyranoside, p-nitrophenyl beta-D-galactopyranoside and arbutin | Arabidopsis thaliana | ? | - |
? | |
n-decyl-beta-D-glucopyranoside + H2O | 11% relative activity | Arabidopsis thaliana | n-decanol + beta-D-glucopyranose | - |
? | |
n-heptyl-beta-D-glucopyranoside + H2O | 17% relative activity | Arabidopsis thaliana | n-heptanol + beta-D-glucopyranose | - |
? | |
n-octyl-beta-D-glucopyranoside + H2O | 7% relative activity | Arabidopsis thaliana | n-octanol + beta-D-glucopyranose | - |
? | |
o-nitrophenyl-beta-D-fucopyranoside + H2O | 117% relative activity | Arabidopsis thaliana | o-nitrophenol + beta-D-fucopyranose | - |
? | |
o-nitrophenyl-beta-D-galactopyranoside + H2O | 17% relative activity | Arabidopsis thaliana | o-nitrophenol + beta-D-galactopyranose | - |
? | |
p-nitrophenyl-beta-D-fucopyranoside + H2O | 71% relative activity | Arabidopsis thaliana | p-nitrophenol + beta-D-fucopyranose | - |
? | |
prunasin + H2O | 39% relative activity | Arabidopsis thaliana | D-mandelonitrile + beta-D-glucose | - |
? | |
salicin + H2O | 4.4% relative activity | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE, recombinant mutated beta-glucosidase | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
beta-D-glucoside glucohydrolase | - |
Arabidopsis thaliana |
beta-glucosidase | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
mutated recombinant beta-glucosidase | Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
is fairly stable in 50 mM sodium acetate buffer, pH 5.6, at temperatures up to 42°C, while retaining only 48% of the original activity at 50°C for 10 min. It is completely inactivated upon incubation at 60°C for 10 min | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
recombinant mutated beta-glucosidase | Arabidopsis thaliana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | mutated recombinant beta-glucosidase is fairly stable at pH 5.0 and 8.5, exhibiting 60 and 62% activities, respectively. It retains over 50% of the original activity between pH 4-7.5. Between pH values 6.0 and 7.2, it retains over 98% activity after incubation at 37°C for 2 h | Arabidopsis thaliana |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
gluconolactone | with para-nitrophenyl-beta-D-glucopyranoside as substrate | Arabidopsis thaliana | |
2.2 | - |
gluconolactone | with ortho-nitrophenyl-beta-D-glucopyranoside as substrate | Arabidopsis thaliana | |
4.7 | - |
gluconolactone | with 4-methylumbelliferyl-beta-D-glucopyranoside as substrate | Arabidopsis thaliana |