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Literature summary for 3.2.1.21 extracted from

  • Turan, Y.
    A pseudo-beta-glucosidase in Arabidopsis thaliana: correction by site-directed mutagenesis, heterologous expression, purification, and characterization (2008), Biochemistry (Moscow), 73, 912-919.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
native and mutated cDNA sequences cloned into vector pPICZalphaB and expressed in Pichia pastoris Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
gluconolactone competitively inhibits with p-nitrophenyl-beta-D-glucopyranoside, o-nitrophenyl-beta-D-glucopyranoside, and 4-methylumbelliferyl-beta-D-glucopyranoside as substrates Arabidopsis thaliana
additional information the enzyme is very tolerant to glucose inhibition Arabidopsis thaliana
p-nitrophenyl-1-thio-beta-D-glucopyranoside competitively inhibits with p-nitrophenyl-beta-D-glucopyranoside, o-nitrophenyl-beta-D-glucopyranoside, and 4-methylumbelliferyl-beta-D-glucopyranoside as substrates Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.78
-
4-methylumbelliferyl-beta-D-glucopyranoside
-
Arabidopsis thaliana
1.9
-
p-nitrophenyl-beta-D-glucopyranoside
-
Arabidopsis thaliana
2.1
-
o-nitrophenyl-beta-D-glucopyranoside
-
Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, SDS-PAGE, recombinant mutated beta-glucosidase Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SLA0
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant beta-glucosidase purified by gel filtration to apparent homogeneity, 21fold with an overall enzyme yield of 6.8% Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.34
-
21fold purified recombinant beta-glucosidase Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-glucopyranoside + H2O 95% relative activity Arabidopsis thaliana 2-nitrophenol + D-glucopyranose
-
?
4-methylumbelliferyl-beta-D-glucopyranoside + H2O 163% relative activity Arabidopsis thaliana 4-methylumbelliferol + beta-D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O 100% relative activity Arabidopsis thaliana 4-nitrophenol + D-glucopyranose
-
?
amygdalin + H2O 48% relative activity Arabidopsis thaliana prunasin + D-glucose
-
?
beta-gentiobiose + H2O 6.3% relative activity Arabidopsis thaliana ?
-
?
D(+)cellobiose + H2O 6.3% relative activity Arabidopsis thaliana ?
-
?
additional information necessity of the nucleophilic glutamic acid residue, located in the motif YITENG, for the substrate hydrolysis. The mutated recombinant beta-glucosidase isoenzyme shows no activity with p-nitrophenyl 1-thio-beta-D-glucopyranoside, p-nitrophenyl beta-D-mannopyranoside, p-nitrophenyl beta-D-galactopyranoside and arbutin Arabidopsis thaliana ?
-
?
n-decyl-beta-D-glucopyranoside + H2O 11% relative activity Arabidopsis thaliana n-decanol + beta-D-glucopyranose
-
?
n-heptyl-beta-D-glucopyranoside + H2O 17% relative activity Arabidopsis thaliana n-heptanol + beta-D-glucopyranose
-
?
n-octyl-beta-D-glucopyranoside + H2O 7% relative activity Arabidopsis thaliana n-octanol + beta-D-glucopyranose
-
?
o-nitrophenyl-beta-D-fucopyranoside + H2O 117% relative activity Arabidopsis thaliana o-nitrophenol + beta-D-fucopyranose
-
?
o-nitrophenyl-beta-D-galactopyranoside + H2O 17% relative activity Arabidopsis thaliana o-nitrophenol + beta-D-galactopyranose
-
?
p-nitrophenyl-beta-D-fucopyranoside + H2O 71% relative activity Arabidopsis thaliana p-nitrophenol + beta-D-fucopyranose
-
?
prunasin + H2O 39% relative activity Arabidopsis thaliana D-mandelonitrile + beta-D-glucose
-
?
salicin + H2O 4.4% relative activity Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE, recombinant mutated beta-glucosidase Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
beta-D-glucoside glucohydrolase
-
Arabidopsis thaliana
beta-glucosidase
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
mutated recombinant beta-glucosidase Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
is fairly stable in 50 mM sodium acetate buffer, pH 5.6, at temperatures up to 42°C, while retaining only 48% of the original activity at 50°C for 10 min. It is completely inactivated upon incubation at 60°C for 10 min Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
recombinant mutated beta-glucosidase Arabidopsis thaliana

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8.5 mutated recombinant beta-glucosidase is fairly stable at pH 5.0 and 8.5, exhibiting 60 and 62% activities, respectively. It retains over 50% of the original activity between pH 4-7.5. Between pH values 6.0 and 7.2, it retains over 98% activity after incubation at 37°C for 2 h Arabidopsis thaliana

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2
-
gluconolactone with para-nitrophenyl-beta-D-glucopyranoside as substrate Arabidopsis thaliana
2.2
-
gluconolactone with ortho-nitrophenyl-beta-D-glucopyranoside as substrate Arabidopsis thaliana
4.7
-
gluconolactone with 4-methylumbelliferyl-beta-D-glucopyranoside as substrate Arabidopsis thaliana