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Literature summary for 3.2.1.21 extracted from

  • Bhiri, F.; Chaabouni, S.E.; Limam, F.; Ghrir, R.; Marzouki, N.
    Purification and biochemical characterization of extracellular beta-glucosidases from the hypercellulolytic Pol6 mutant of Penicillium occitanis (2008), Appl. Biochem. Biotechnol., 149, 169-182.
    View publication on PubMed
Search for more literature for 3.2.1.21

Protein Variants

Protein Variants Comment Organism
additional information isolation of isoforms beta-glu 1 and beta-glu 2 from hypercellulolytic Pol6 mutant Penicillium sp. 'occitanis'

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 2 mM, significant inhibition, both isoforms beta-glu 2 and beta-glu 1 Penicillium sp. 'occitanis'
D-glucose competitive, both isoforms beta-glu 2 and beta-glu 1 Penicillium sp. 'occitanis'
delta-gluconolactone competitive, both isoforms beta-glu 2 and beta-glu 1 Penicillium sp. 'occitanis'
Hg2+ 2 mM, significant inhibition, both isoforms beta-glu 2 and beta-glu 1 Penicillium sp. 'occitanis'

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
 cellobiose both isoforms beta-glu 2 and beta-glu 1 Penicillium sp. 'occitanis'
1.43
-
 4-nitrophenyl beta-D-glucopyranoside both isoforms beta-glu 2 and beta-glu 1 Penicillium sp. 'occitanis'

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98000
-
 x * 98000, SDS-PAGE, both isoforms beta-glu 1 and beta-glu 2 from hypercellulolytic Pol6 mutant Penicillium sp. 'occitanis'

Organism

Organism UniProt Comment Textmining
Penicillium sp. 'occitanis'
-
 isolation of isoforms beta-glu 1 and beta-glu 2 from hypercellulolytic Pol6 mutant
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 8% carbohydrate content, both isoforms beta-glu 1 and beta-glu 2 from hypercellulolytic Pol6 mutant Penicillium sp. 'occitanis'

Purification (Commentary)

Purification (Comment) Organism
isolation of isoforms beta-glu 1 and beta-glu 2 from hypercellulolytic Pol6 mutant Penicillium sp. 'occitanis'

Source Tissue

Source Tissue Comment Organism Textmining
culture fluid
-
 Penicillium sp. 'occitanis'
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
103
-
 substrate 4-nitrophenyl beta-D-glucopyranoside, pH 4.8, 60°C, isoform beta-glu 2 Penicillium sp. 'occitanis'
173
-
 substrate 4-nitrophenyl beta-D-glucopyranoside, pH 4.8, 60°C, isoform beta-glu 2 Penicillium sp. 'occitanis'

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Penicillium sp. 'occitanis' 4-nitrophenol + beta-D-glucose
-
 ?
cellobiose + H2O
-
 Penicillium sp. 'occitanis' 2 beta-D-glucose
-
 ?
additional information in presence of high concentrations of glucose, both isoforms show trans-glycosidase activity Penicillium sp. 'occitanis' ?
-
 ?

Subunits

Subunits Comment Organism
? x * 98000, SDS-PAGE, both isoforms beta-glu 1 and beta-glu 2 from hypercellulolytic Pol6 mutant Penicillium sp. 'occitanis'

pI Value

Organism Comment pI Value Maximum pI Value
Penicillium sp. 'occitanis' isoelectric focusing, isoform beta-glu 2
-
 5.3
Penicillium sp. 'occitanis' isoelectric focusing, isoform beta-glu 1
-
 5.5